FastQCFastQC Report
Tue 7 May 2024
EGAF00007823166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019
Total Bases304.8 kbp
Sequences flagged as poor quality0
Sequence length151
%GC63

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1889.311540366518079No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC914.507181773155027No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC321.58494304110946No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.8915304606240713No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGG170.8420009905894007No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGG130.6438831104507181No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGG110.5448241703813769No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGGGGGGG100.49529470034670625No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGG90.44576523031203563No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGGGGGGG90.44576523031203563No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGG80.396235760277365No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTGGGGGGGGGGG80.396235760277365No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGT80.396235760277365No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGGGGGGGG80.396235760277365No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTG80.396235760277365No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.3467062902426944No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGG70.3467062902426944No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGG70.3467062902426944No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGG70.3467062902426944Illumina RNA PCR Primer (95% over 21bp)
TGGTATGGCTGTGTGGGGTTCCTGGGGCCTTGCCAAAATGTCGGTACACC70.3467062902426944No Hit
GACATAGGGTTTGGTATGGCTGTGTGGGGTTCCTGGGGCCTTGCCAAAAT70.3467062902426944No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGGGGG60.2971768202080238No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGGGGGGGG60.2971768202080238No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGCGG60.2971768202080238No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGG60.2971768202080238No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGG60.2971768202080238No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG50.24764735017335313No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG50.24764735017335313No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA50.24764735017335313No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGGGGG40.1981178801386825No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTC40.1981178801386825No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGGGG40.1981178801386825No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGGG40.1981178801386825No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGGGGGGGGGG40.1981178801386825No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT40.1981178801386825No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGGGGGGGGGG40.1981178801386825No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGCGGGGGGGGGG40.1981178801386825No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGGGGG40.1981178801386825No Hit
GGTTGAGAGCGCTTATTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGT40.1981178801386825No Hit
GCCCTATACGTCGTCTTGAAGCCGACTTAGCAGAGCCCTGTGTTTTTGCT40.1981178801386825No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGGGGGGG40.1981178801386825No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTGGG40.1981178801386825No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTGGGGGG40.1981178801386825No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGGTGGTCGCC40.1981178801386825Illumina RNA PCR Primer (96% over 29bp)
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTC40.1981178801386825No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGG30.1485884101040119No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTG30.1485884101040119No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGTGGG30.1485884101040119No Hit
GGTTTGGTATGGCTGTGTGGGGTTCCTGGGGCCTTGCCAAAATGTCGGTA30.1485884101040119No Hit
GTTAAGATCCAGGGTTAGTTTTTGTAGCCTCTGCTGGCCCTTCGGCCTCT30.1485884101040119No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT30.1485884101040119No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCT30.1485884101040119No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTCGG30.1485884101040119No Hit
CTCTTGTCCTTTCGTACTGGGAGAAATCGTAAATAGATAGAAACCGACCT30.1485884101040119No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTG30.1485884101040119No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA30.1485884101040119No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTGGG30.1485884101040119No Hit
CGTATATATTTTATTTAGATTTTATTCATAAATTAGGTTGAGAGCGCTTA30.1485884101040119No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1485884101040119No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGG30.1485884101040119No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTG30.1485884101040119No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGGGGGGGGGGGG30.1485884101040119No Hit
GCATACGACTTTGTCGAAGGCGATCAGGCTAACCGGGTCGATACCAGTTG30.1485884101040119No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGGGGGGGGGGGGG30.1485884101040119No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1485884101040119No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACC30.1485884101040119No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGG30.1485884101040119No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGGG30.1485884101040119No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA30.1485884101040119No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA30.1485884101040119No Hit
CCGTTTACCTGGGCTTCATTTCGGAGCTTGCACCCCTCCACTTAACCTTC30.1485884101040119No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTCGGGGG30.1485884101040119No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)