FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1579
Sequences flagged as poor quality0
Sequence length151
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT17411.019632678910703No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC674.243191893603546No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGG271.7099430018999366No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC191.2032932235592146No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGGGGGGG150.9499683343888538No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGT140.8866371120962635No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGGGGGGG140.8866371120962635No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.8233058898036731No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGG130.8233058898036731No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGG110.6966434452184928No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGG100.6333122229259025No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGG90.5699810006333123No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGG80.506649778340722Illumina RNA PCR Primer (95% over 21bp)
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGGGGGGGG80.506649778340722No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGG70.44331855604813175No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGGGGGGGG60.37998733375554145No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGGGGGGGGGG60.37998733375554145No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGG50.31665611146295125No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.31665611146295125No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGG50.31665611146295125No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGGGGGGGGGGGGG50.31665611146295125No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGG50.31665611146295125No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTG50.31665611146295125No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGTGGGGGGGGGGG50.31665611146295125No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGGG40.253324889170361No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGG40.253324889170361No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTG40.253324889170361No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTCGG40.253324889170361No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTGGGGGGGGGGG40.253324889170361No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTGGG40.253324889170361No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGTGGGGGGGGGGG40.253324889170361No Hit
GTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.253324889170361No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGGTGGTCGCC40.253324889170361Illumina RNA PCR Primer (96% over 29bp)
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGC40.253324889170361No Hit
GGTTTCGGGTCTAATGCAACAAACTCAGTCGCCCTATTCAGACTCGCTTT30.18999366687777072No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGGGGGGGGGGGGG30.18999366687777072No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGGGGG30.18999366687777072No Hit
CCTTCAACCTGGTCATGCCTAGATCACCGGGTTTCGGGTCTAATGCAACA30.18999366687777072No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTGGTGGGGGGGGGGG30.18999366687777072No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGTG30.18999366687777072No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTGGGGGGGGGGG30.18999366687777072No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGGGGGGGG30.18999366687777072No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGCGG30.18999366687777072No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGG30.18999366687777072No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGG30.18999366687777072No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTG30.18999366687777072No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGTGGGGGGG30.18999366687777072No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGGGGGGGGGGGGG30.18999366687777072No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGCGGGGGGGGGG30.18999366687777072No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGCGGGGGGGGGG30.18999366687777072No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGTATATCTCGGTGGTCGCC30.18999366687777072Illumina Single End PCR Primer 1 (95% over 21bp)
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGGG30.18999366687777072No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG30.18999366687777072No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTCGGGGGGGGGG30.18999366687777072No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG30.18999366687777072No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTGGTGGG30.18999366687777072No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGG30.18999366687777072No Hit
GCTTAAGCTTGCTCGTAACTTTAAGTCGCTGACCCATTATACAAAAGGTA30.18999366687777072No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGGTGG20.1266624445851805Illumina RNA PCR Primer (95% over 24bp)
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGGGGG20.1266624445851805No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGCGGGG20.1266624445851805No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGTCTCTTATACAC20.1266624445851805No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTG20.1266624445851805No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTC20.1266624445851805No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTC20.1266624445851805No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGG20.1266624445851805No Hit
GTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1266624445851805No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTCGGGGG20.1266624445851805No Hit
CATCTGCTGGAAGGTGGACAGTGAGGCCAGGATGGAGCCACCGATCCACA20.1266624445851805No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20.1266624445851805No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTG20.1266624445851805No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGGGGGTGGGG20.1266624445851805No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGCGGGGG20.1266624445851805No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGGGGG20.1266624445851805No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTCGGGGGGGGGG20.1266624445851805No Hit
CATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1266624445851805No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTAGG20.1266624445851805No Hit
ACACAGAGTACTTGCGCTCAGGAGGAGCAATGATCTTGATCTTCATGGTG20.1266624445851805No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGGGGGGGG20.1266624445851805No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTC20.1266624445851805No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTTGGTGTGGG20.1266624445851805No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTG20.1266624445851805No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1266624445851805No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTGGGTGGTGGGG20.1266624445851805No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTG20.1266624445851805No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGCGGGGGGGGGGGG20.1266624445851805No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA20.1266624445851805No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGA20.1266624445851805No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGTG20.1266624445851805No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGGG20.1266624445851805No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1266624445851805No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGGGGGGGGTGGGG20.1266624445851805No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTC20.1266624445851805No Hit
GCATAGCAGATGCAAATGAAGGGACTTGGGGCTAGTCAGGAAGAAAGGGA20.1266624445851805No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGGGGGGGGGGGG20.1266624445851805No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1266624445851805No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTTGTGGTGGG20.1266624445851805No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA20.1266624445851805No Hit
GAATTGGTAGATCTCGCGACCCCCGCATCCATCCAGTGCTCTACCCCCCA20.1266624445851805No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC20.1266624445851805No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGGG20.1266624445851805No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATATCGGTGG20.1266624445851805No Hit
GTATAAGAGACAGATTGCGCAATGCATCCCCCGGTTAACATTGCGCAATC20.1266624445851805No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGGTGG20.1266624445851805No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGTCGGTGGGGGGC20.1266624445851805No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGGGGGGG20.1266624445851805No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTGGG20.1266624445851805No Hit
GATCCACACAGAGTACTTGCGCTCAGGAGGAGCAATGATCTTGATCTTCA20.1266624445851805No Hit
GGCCAGGATGGAGCCACCGATCCACACAGAGTACTTGCGCTCAGGAGGAG20.1266624445851805No Hit
TCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGG20.1266624445851805No Hit
ATCTTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGG20.1266624445851805No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGGGGGTGG20.1266624445851805No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGTGG20.1266624445851805No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)