FastQCFastQC Report
Tue 7 May 2024
EGAF00007823176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7709
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGG2222.8797509404592034No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGT2022.6203139187962123No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1642.1273835776365284No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1121.4528473213127513No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.8820858736541706No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.8172266182384227No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAAC570.7393955117395252No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTT440.5707614476585808No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTG440.5707614476585808No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTG420.5448177454922818No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG400.5188740433259826No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG390.5059021922428331No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTA370.4799584900765339No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG300.389155532494487No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG290.3761836814113374No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGA260.33726812816188867No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTC260.33726812816188867No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC260.33726812816188867No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC230.29835257491244No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA200.2594370216629913No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG190.24646517057984174No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC190.24646517057984174No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA170.22052146841354264No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA170.22052146841354264No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.22052146841354264No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC170.22052146841354264No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC170.22052146841354264No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC160.20754961733039304No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT150.1945777662472435No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGG150.1945777662472435No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCT150.1945777662472435No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGGGGGGGG140.1816059151640939No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.1816059151640939No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA120.15566221299779479No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGG120.15566221299779479No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA120.15566221299779479No Hit
GTATTCACTCGAGGCGCTACCTAAATAGCTTTCGCGGAGAACCAGCTATT120.15566221299779479No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA120.15566221299779479No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGGGGGGGG110.1426903619146452No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGGGGGGG110.1426903619146452No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGG110.1426903619146452No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC110.1426903619146452No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA110.1426903619146452No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAA110.1426903619146452No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.1426903619146452No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAT110.1426903619146452No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGG100.12971851083149566No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTG100.12971851083149566No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA100.12971851083149566No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTC90.1167466597483461No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA90.1167466597483461No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGG90.1167466597483461No Hit
GCCCTAGCCCATCCAGTGCTCTACCCCGCACGGTATTCACTCGAGGCGCT90.1167466597483461No Hit
GCATACGCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG90.1167466597483461No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA90.1167466597483461No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA80.10377480866519652No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTGGGGGGGGGGG80.10377480866519652No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)