FastQCFastQC Report
Tue 7 May 2024
EGAF00007823186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340
Total Bases202.3 kbp
Sequences flagged as poor quality0
Sequence length151
%GC70

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1138.432835820895521No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC564.179104477611941No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGG261.9402985074626864No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC181.3432835820895521No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGG171.2686567164179103No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGG151.1194029850746268No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGT120.8955223880597015No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGGGGGGG110.8208955223880596No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGGGGGGGG100.7462686567164178No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGGGGGGG100.7462686567164178No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGGGGGGGG90.6716417910447761No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTG90.6716417910447761No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGG70.5223880597014926No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGG60.44776119402985076No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.44776119402985076No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGGGGGGGGGGGGG60.44776119402985076No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGGTGGTCGCC60.44776119402985076Illumina RNA PCR Primer (96% over 29bp)
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGG60.44776119402985076No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGGGGG50.3731343283582089No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGG50.3731343283582089No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGG50.3731343283582089No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTGGGGGGGGGGG50.3731343283582089No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGG50.3731343283582089No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGG50.3731343283582089Illumina RNA PCR Primer (95% over 21bp)
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGGG50.3731343283582089No Hit
GGTCTGAATCGTGGTGGGGGTGTTCGGTCCTTCCGGGCCTCGCGGGATCG40.2985074626865672No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTG40.2985074626865672No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTCGG40.2985074626865672No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGTGGGGGGGGGGG40.2985074626865672No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGGGGGGG40.2985074626865672No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTGGG40.2985074626865672No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTG40.2985074626865672No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGGTGG30.22388059701492538Illumina RNA PCR Primer (95% over 24bp)
GTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTG30.22388059701492538No Hit
GTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.22388059701492538No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30.22388059701492538No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGCGG30.22388059701492538No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGTGGGGGGTGGGG30.22388059701492538No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTG30.22388059701492538No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGGGGGGGGGG30.22388059701492538No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGTGGGGGG30.22388059701492538No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGG30.22388059701492538No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTG30.22388059701492538No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGTGGGGGGC30.22388059701492538No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTGGGTGTG30.22388059701492538No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGG30.22388059701492538No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTT30.22388059701492538No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTGGGGGGGGG20.1492537313432836No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGAGTTGGGGGGGGGGGGG20.1492537313432836No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGCGGGGGGGGGG20.1492537313432836No Hit
GTGTATAAGAGACAGATTGCGCACTCTGTCTCTTATACACATCTGACGCT20.1492537313432836No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGTGGG20.1492537313432836No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTC20.1492537313432836No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGTGGGGGGG20.1492537313432836No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1492537313432836No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGGGGGGGC20.1492537313432836No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGTGG20.1492537313432836No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATCTG20.1492537313432836No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGTTGGTGTGGGGGGGGGGG20.1492537313432836No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTTGTTGTC20.1492537313432836No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGTGGGTGGGGGGG20.1492537313432836No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGGGGGGC20.1492537313432836No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTGGGGGGGGGGG20.1492537313432836No Hit
GTCTATATGCAGGAGCTATTTCCTGGAACCGCTTCGCTGCAAGATCAATC20.1492537313432836No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGG20.1492537313432836No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTG20.1492537313432836No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTCGGTGGGGGGG20.1492537313432836No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTG20.1492537313432836No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAATTTT20.1492537313432836No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1492537313432836No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACCATCTGACGCTGCC20.1492537313432836No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1492537313432836No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTAGG20.1492537313432836No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTGGTCGGTGGGGGGG20.1492537313432836No Hit
GTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1492537313432836No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGCGGGGGGGGGG20.1492537313432836No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTCGGGG20.1492537313432836No Hit
GTTTAGGGTACGGTCTATATGCAGGAGCTATTTCCTGGAACCGCTTCGCT20.1492537313432836No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGTCGGGGGGGGGG20.1492537313432836No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGCGG20.1492537313432836No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC20.1492537313432836No Hit
CTTTATCGTTACTCATGTCAGCATTCTCACTTCCGATACCTCCAGGATGT20.1492537313432836No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGGG20.1492537313432836No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG20.1492537313432836No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGGGGG20.1492537313432836No Hit
TCGCGGGATCGATTCCTAACAACCTTGCTATGGATGGCACAGCTCACGCA20.1492537313432836No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGGTGGGG20.1492537313432836No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTCGGGGGGGGGG20.1492537313432836No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGTGGGGGGGGGGG20.1492537313432836No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGC20.1492537313432836No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20.1492537313432836No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC20.1492537313432836No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTGGGTGG20.1492537313432836No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTCGGGGG20.1492537313432836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)