FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036
Sequences flagged as poor quality0
Sequence length151
%GC72

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT10410.038610038610038No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC292.799227799227799No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGG131.2548262548262548No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGGGGGGGG131.2548262548262548No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC131.2548262548262548No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGGGGGGG121.1583011583011582No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGG100.9652509652509652No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.7722007722007722No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGGGGGGG80.7722007722007722No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGG70.6756756756756757No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGG60.5791505791505791No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGG60.5791505791505791No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGCGGGGGGGGGG60.5791505791505791No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGGGGGGGGGGGGG60.5791505791505791No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGGTGGTCGCC60.5791505791505791Illumina RNA PCR Primer (96% over 29bp)
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGT50.4826254826254826No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGTGGGGGGGGGGG50.4826254826254826No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGGGGGGGG50.4826254826254826No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTGGGGGGGGGGG50.4826254826254826No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGTGGGGGGGGGGG50.4826254826254826No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGG50.4826254826254826No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGGGGGGGGGG50.4826254826254826No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGGGGGGGGGGGGG40.3861003861003861No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGGGGG40.3861003861003861No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGGGGGGGGGG40.3861003861003861No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGG40.3861003861003861No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGGG40.3861003861003861No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTCGGGGGGGGGG40.3861003861003861No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTGGGGGGGGGGG40.3861003861003861No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGGGGGGC40.3861003861003861No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTCGGGGGGGGGG30.28957528957528955No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGTGGGGGG30.28957528957528955No Hit
GTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.28957528957528955No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTCGGGGG30.28957528957528955No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGCGGGGGGGGGGGG30.28957528957528955No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGGTGGGG30.28957528957528955No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGG30.28957528957528955No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGTGGGTGTGGGGGGGGGGG30.28957528957528955No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGGG30.28957528957528955No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATATCGGTGGTCGCC30.28957528957528955Illumina Single End PCR Primer 1 (95% over 21bp)
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGGGGG30.28957528957528955No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGTGGGGGGG30.28957528957528955No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACCATCTGACGCTGCC30.28957528957528955No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGGTGG20.19305019305019305Illumina RNA PCR Primer (95% over 24bp)
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGG20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGTGGGGGGG20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGGCGGGGGGGGGG20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTCTGGGGGGGGGGG20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTGGGTGGGGGGG20.19305019305019305No Hit
TATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGG20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTGTGGGGTGGGGGGG20.19305019305019305No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGGGG20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGCGGGGGGGGGC20.19305019305019305No Hit
GTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19305019305019305No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATAACACATCTGACGC20.19305019305019305No Hit
NTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT20.19305019305019305No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGG20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGTGGGGGGG20.19305019305019305No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20.19305019305019305No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGGTGGTGGGG20.19305019305019305No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA20.19305019305019305No Hit
GACTCGCTTTCGCTGCGCCTACACCTACCGGCTTAAGCTTGCTCGATACA20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGTGGGTGGGGGGG20.19305019305019305No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGCGGGGG20.19305019305019305No Hit
GTATAAGAGACAGATTGCGCAATGCATCCCCAGGATTTCCATTGCGCAAT20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGTGGGGCGGGGGGGGGGGG20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGCGGGGGGGGGG20.19305019305019305No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGGGGGGG20.19305019305019305No Hit
GTATAAGAGACAGATTGCGCAATGTGGTCCCCGGGACTCCATTGCGCAAT20.19305019305019305No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGGGGGGGG20.19305019305019305No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTGGGGGG20.19305019305019305No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC20.19305019305019305No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT20.19305019305019305No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTCGG20.19305019305019305No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTG20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGGCGGGGGTGGGG20.19305019305019305No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTG20.19305019305019305No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGGAGG20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGGTGGGG20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTCGGGGGGGGGG20.19305019305019305No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTCGGGGGGGGGG20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTGGTGGGTGGGGGGG20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTCTGGGGGGGGGGG20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGGTGGGGGGG20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGAGGGGGTGGGG20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGTGGTTGGGGGGGGTGGGG20.19305019305019305No Hit
ATCTTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGG20.19305019305019305No Hit
GTATAAGAGACAGATTGCGCAATGAGACCCCCGAGCGGCATTGCGCAATC20.19305019305019305No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGCGGGGGGGGGGGG20.19305019305019305No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)