FastQCFastQC Report
Tue 7 May 2024
EGAF00007823190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733
Total Bases412.6 kbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1676.110501280643981No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC812.96377607025247No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGG351.2806439809732895No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGT341.2440541529454812No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.804976216611782No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.6220270764727406No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG140.5122575923893158No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC140.5122575923893158No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAAC110.402488108305891No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC110.402488108305891No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGGGGGGG100.3658982802780827No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG100.3658982802780827No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTC100.3658982802780827No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGGGGGGG100.3658982802780827No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTG90.3293084522502744No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG90.3293084522502744No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC90.3293084522502744No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG80.29271862422246614No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGG80.29271862422246614No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGG70.2561287961946579No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA70.2561287961946579No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGGG70.2561287961946579No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGG60.21953896816684962No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGG60.21953896816684962No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGGGGGGGGGGGGG60.21953896816684962No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTC60.21953896816684962No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.21953896816684962No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.21953896816684962No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAT60.21953896816684962No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGGGGGGGG50.18294914013904134No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGG50.18294914013904134No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGA50.18294914013904134No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTA50.18294914013904134No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC50.18294914013904134No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA50.18294914013904134No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGGGGGGGG50.18294914013904134No Hit
TCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGG50.18294914013904134No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGGGGGGGGGG40.14635931211123307No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA40.14635931211123307No Hit
CAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.14635931211123307No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTG40.14635931211123307No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA40.14635931211123307No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGGG40.14635931211123307No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC40.14635931211123307No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA40.14635931211123307No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGG40.14635931211123307No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTG40.14635931211123307No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTGGGGGGGGGGG40.14635931211123307No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGG40.14635931211123307Illumina RNA PCR Primer (95% over 21bp)
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGTGGGGGGGGGGG40.14635931211123307No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA40.14635931211123307No Hit
TCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA40.14635931211123307No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGGGGG30.10976948408342481No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGG30.10976948408342481No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA30.10976948408342481No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAA30.10976948408342481No Hit
TGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTG30.10976948408342481No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.10976948408342481No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA30.10976948408342481No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTG30.10976948408342481No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG30.10976948408342481No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTG30.10976948408342481No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGCTGGG30.10976948408342481No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTGGGTGGGGGGG30.10976948408342481No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGG30.10976948408342481No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA30.10976948408342481No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGGG30.10976948408342481No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGCGG30.10976948408342481No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGTGGGGGG30.10976948408342481No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAT30.10976948408342481No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.10976948408342481No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA30.10976948408342481No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGTGGGGGGG30.10976948408342481No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGGGGGGGGGGGG30.10976948408342481No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTC30.10976948408342481No Hit
TACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.10976948408342481No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA30.10976948408342481No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGGTGGTCGCC30.10976948408342481Illumina RNA PCR Primer (96% over 29bp)
TTTTTTTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG30.10976948408342481No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA30.10976948408342481No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)