FastQCFastQC Report
Tue 7 May 2024
EGAF00007823191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8803
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3904.430307849596728No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2753.123935022151539No Hit
ATTGCGCAATGCTAAGCGGGGGGAGTAGGGCGAGGCACGTGAAACCTTGT1281.4540497557650802No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCG860.9769396796546632No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCG710.8065432239009429No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.46575031239350223No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG350.3975917300920141No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACC330.3748722026581847No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGG330.3748722026581847No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG310.35215267522435534No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT290.32943314779052596No Hit
ATTGCGCAATGACTAGGGTGGGTCACTTGAGGTCAGGAGTTCAAAAACAG270.3067136203566966No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCT270.3067136203566966No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG270.3067136203566966No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC270.3067136203566966No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCT240.2726343292059525No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCAT240.2726343292059525No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCAGCG200.22719527433829376No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG190.21583551062137907No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT180.20447574690446438No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT180.20447574690446438No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT170.19311598318754972No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATGG170.19311598318754972No Hit
ATTGCGCAATGTCGGTTCAGGGGACGCAGCGAAAGGTGCCAAATAGATAG160.18175621947063503No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCA160.18175621947063503No Hit
ATCTGTGGCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCG150.1703964557537203No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC150.1703964557537203No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC140.15903669203680562No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA130.14767692831989093No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120.13631716460297624No Hit
ATTGCGCAATGCATGTTATGGGGAGCGAAGCGGGAAGAGCTCAAATTTGA120.13631716460297624No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC100.11359763716914688No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT90.10223787345223219No Hit
ATTGCGCAATGCTCGATTCGGGGTGGCGGCGACGACCCATTCGAACGTCT90.10223787345223219No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT90.10223787345223219No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG90.10223787345223219No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)