FastQCFastQC Report
Tue 7 May 2024
EGAF00007823194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171
Total Bases176.8 kbp
Sequences flagged as poor quality0
Sequence length151
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT17214.688300597779675No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC716.063193851409053No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC151.2809564474807857No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGG121.0247651579846286No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGGTGGTCGCC121.0247651579846286Illumina RNA PCR Primer (96% over 29bp)
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGG110.9393680614859093No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGG90.7685738684884714No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGGGGGGG90.7685738684884714No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGGGGGGGGGG90.7685738684884714No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGGGGGGGG80.6831767719897524No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGG80.6831767719897524No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGGGGGGG70.5977796754910333No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGGGGGGGGGGGGG60.5123825789923143No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGGGGGGGGGG60.5123825789923143No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGG60.5123825789923143No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.4269854824935952No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGGGGGGG50.4269854824935952No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.4269854824935952No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTG50.4269854824935952No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGG40.3415883859948762No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGGGGGGG40.3415883859948762No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGG40.3415883859948762Illumina RNA PCR Primer (95% over 21bp)
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGGGGGGGG40.3415883859948762No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGGG40.3415883859948762No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGG40.3415883859948762No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGG40.3415883859948762No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTGGGGGGGGGGG40.3415883859948762No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGGTGG30.25619128949615716Illumina RNA PCR Primer (95% over 24bp)
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGT30.25619128949615716No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.25619128949615716No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGG30.25619128949615716No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGTGGGGGG30.25619128949615716No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGGGGG30.25619128949615716No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTCGGGGG30.25619128949615716No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30.25619128949615716No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTGGTGGGGGGGGGGG30.25619128949615716No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGTG30.25619128949615716No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTGGGGGG30.25619128949615716No Hit
TATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTGGGGG30.25619128949615716No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGGTGGGGGGGGGGG30.25619128949615716No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTGGGGGG30.25619128949615716No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTGGGGGGGGGGG30.25619128949615716No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTCGGGGGGGGGG20.1707941929974381No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGGGGGGGG20.1707941929974381No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTCGCGGTGGGGGGG20.1707941929974381No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGGG20.1707941929974381No Hit
GTAGTACTCCCCGGTATTCGGAGTTTGATTAGGATCAGTAAGACGGTGAG20.1707941929974381No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGCGGGGGGGGGG20.1707941929974381No Hit
GGTCAGGGTTGTTGCCCTCTCCACGACGGACGTTAGCACCCGCCGTGTGT20.1707941929974381No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTG20.1707941929974381No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTGGGTGGGGGGG20.1707941929974381No Hit
GTGTATAAGAGACAGATTGCGCAATGAGCCCCGGCGGACTCATTGCGCAA20.1707941929974381No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGCGGGGGGGGGG20.1707941929974381No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTGGGGGGGGGGGGGG20.1707941929974381No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1707941929974381No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGAGG20.1707941929974381No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTC20.1707941929974381No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGTA20.1707941929974381No Hit
ATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG20.1707941929974381No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT20.1707941929974381No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGCGG20.1707941929974381No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGGCGCGGGGG20.1707941929974381No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGGCGGGGGGGGGG20.1707941929974381No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTGGG20.1707941929974381No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGTG20.1707941929974381No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATATCGGTGGTCGCC20.1707941929974381Illumina Single End PCR Primer 1 (95% over 21bp)
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAGTGTA20.1707941929974381No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGGGGG20.1707941929974381No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTG20.1707941929974381No Hit
CTTAGATGGTGGTCAGGGTTGTTGCCCTCTCCACGACGGACGTTAGCACC20.1707941929974381No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGTTGGAGG20.1707941929974381No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGGGGG20.1707941929974381No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTCTCGGTGGGGGGG20.1707941929974381No Hit
ATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTGGGGGGGGGGGG20.1707941929974381No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGGAGGGGGGGGGG20.1707941929974381No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGTGTCGGGGGGGGGG20.1707941929974381No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA20.1707941929974381No Hit
GCACCCGCCGTGTGTCTGCCCAGTAGTACTCCCCGGTATTCGGAGTTTGA20.1707941929974381No Hit
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGTGGTGTGGGTGG20.1707941929974381No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC20.1707941929974381No Hit
TCTGACGCTGCCGACGAGGCTCAGAACGTGTAGATCTCGGTGGTCGCCGT20.1707941929974381Illumina RNA PCR Primer (96% over 31bp)
ATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGGCGGGGG20.1707941929974381No Hit
CTCCACGACGGACGTTAGCACCCGCCGTGTGTCTGCCCAGTAGTACTCCC20.1707941929974381No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGGGGTG20.1707941929974381No Hit
CATCTGACGCTGCCGACGAGGCTCAGAACGTGGTTTTGTGGGGGGGGGGG20.1707941929974381No Hit
CTTATACACATCTGACGCTGCCGACGAGGCTCAGAACGTGGGGTTGTGGG20.1707941929974381No Hit
GTATAAGAGACCGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC20.1707941929974381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)