FastQCFastQC Report
Tue 7 May 2024
EGAF00007823198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4339
Total Bases655.1 kbp
Sequences flagged as poor quality0
Sequence length151
%GC61

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT54412.53745102558193No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2435.600368748559577No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC631.4519474533302605No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGT390.8988246139663517No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTA200.46093569946992397No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTT160.36874855957593916No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.34570177460244295No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGA140.32265498962894673No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.27656141968195436No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG110.2535146347084582No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG100.23046784973496198No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAG100.23046784973496198No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGGGGGGGGG90.20742106476146577No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA80.18437427978796958No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTG80.18437427978796958No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGG80.18437427978796958No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC80.18437427978796958No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTCGGGGGGGGGG80.18437427978796958No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC80.18437427978796958No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG70.16132749481447337No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC70.16132749481447337No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC70.16132749481447337No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGGGGGGGGGGGGG60.13828070984097718No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA60.13828070984097718No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.13828070984097718No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGTGGGGGGGGGGG60.13828070984097718No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGGTGGTCGCC60.13828070984097718Illumina RNA PCR Primer (95% over 23bp)
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGTCGG50.11523392486748099No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGGGGGGGGGG50.11523392486748099No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTTTTGTGGG50.11523392486748099No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGGGGGGGGGGGGG50.11523392486748099No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG50.11523392486748099No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC50.11523392486748099No Hit
GTGTATAAGTGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT50.11523392486748099No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTG50.11523392486748099No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA50.11523392486748099No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA50.11523392486748099No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.11523392486748099No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGG50.11523392486748099No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGTGGGGGGG50.11523392486748099No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGG50.11523392486748099No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)