FastQCFastQC Report
Tue 7 May 2024
EGAF00007823199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16844
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAATCG8384.97506530515317No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAATCT5713.389931132747566No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2651.5732605081928284No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2281.353597720256471No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2001.1873664212776063No Hit
ATTGCGCAATGTTGACGGCGGGGGGTCTGATTTGATCGGAGCTGGGCTTC1580.9380194728093089No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGA1130.6708620280218476No Hit
ATTGCGCAATGTCTTCCACGGGGGGAAAAACGTGAGATGCGGTGGAGAGA980.581809546426027No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAAT930.5521253858940869No Hit
ATTGCGCAATGATATATGGGGGGAATAACTCAGGGAAACTTGTGCTAATA910.5402517216813109No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG800.47494656851104256No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC790.46900973640465443No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC770.4571360721918784No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT710.4215150795535502No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAC620.36808359059605794No Hit
ATTGCGCAATGTGTCTAAGGGGGGAATTGACGGGGGCCTGCACAAGCGGT600.35620992638328186No Hit
ATTGCGCAATGTAAGGGTGGGGGAACTCGGCAAATTGCCTCCGTACCTTC530.31465210163856566No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT530.31465210163856566No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT520.30871526953217765No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAATCG500.29684160531940157No Hit
ATTGCGCAATGTTGTCTAAGGGGGGAATTGACGGGGGCCTGCACAAGCGG490.2909047732130135No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA470.2790311090002375No Hit
ATTGCGCAATGGGCGGCGGGGGGGGTCTGATTTGATCGGAGCTGGGCTTC420.24934694846829733No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC390.2315364521491332No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT370.21966278793635716No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA340.20185229161719304No Hit
ATTGCGCAATGACAAGCGGGGGGGGTCTGATTTGATCGGAGCTGGGCTTC320.189978627404417No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAATCT310.18404179529802897No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT290.17216813108525292No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG290.17216813108525292No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACC280.16623129897886488No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG270.16029446687247684No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT260.15435763476608882No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG260.15435763476608882No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAAGCG250.14842080265970078No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC250.14842080265970078No Hit
ATTGCGCAATGATCGTGCAGGGGACCGTAGTGGGTGATAGTCCCGTATAC240.14248397055331277No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC240.14248397055331277No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAATCTCG230.13654713844692473No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA230.13654713844692473No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAC230.13654713844692473No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG230.13654713844692473No Hit
ATTGCGCAATGAGTGCCTAGGGGACCGTACCCTAAACCGACACAGGTGGG220.1306103063405367No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAATCGCG220.1306103063405367No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAAATGG220.1306103063405367No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGA200.11873664212776064No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT190.1127998100213726No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG190.1127998100213726No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT190.1127998100213726No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG180.10686297791498457No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT170.10092614580859652No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACCATGAACTCG170.10092614580859652No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC170.10092614580859652No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)