FastQCFastQC Report
Tue 7 May 2024
EGAF00007823200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16844
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTA14938.863690334837331No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3492.0719544051294227No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2571.525765851341724No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.7836618380432201No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.7302303490857279No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACA1220.7242935169793397No Hit
CTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGG1120.6649251959154595No Hit
ATCTACAACAGCGGGTGCAGGCGCACCATCCTAAATAGGTATCATTATGC990.5877463785324151No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTG870.5165043932557587No Hit
CTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTC850.5046307290429827No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC760.4511992400854904No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAA660.39183091902161005No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA600.35620992638328186No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG530.31465210163856566No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG500.29684160531940157No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC490.2909047732130135No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACTGTGTA450.2671574447874614No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.2434101163619093No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA380.2255996200427452No Hit
CATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGGTGGTCGCC370.21966278793635716Illumina Single End PCR Primer 1 (100% over 22bp)
CTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGA360.21372595582996914No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC360.21372595582996914No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGGGTA330.19591545951080502No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA330.19591545951080502No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG330.19591545951080502No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC320.189978627404417No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.16623129897886488No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA270.16029446687247684No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG270.16029446687247684No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAA260.15435763476608882No Hit
ATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGGTGG250.14842080265970078No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTT250.14842080265970078No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGGA240.14248397055331277No Hit
CTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGTTCTCGG230.13654713844692473No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT220.1306103063405367No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC220.1306103063405367No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGA210.12467347423414867No Hit
ATCCTAAATAGGTATCATTATGCATATGTATCGACCAATGCGTCGCGCAG210.12467347423414867No Hit
CTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGTTCTC200.11873664212776064No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG200.11873664212776064No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC200.11873664212776064No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAG200.11873664212776064No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA200.11873664212776064No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGT200.11873664212776064No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA200.11873664212776064No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.11873664212776064No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC180.10686297791498457No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA180.10686297791498457No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC180.10686297791498457No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCAC170.10092614580859652No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT170.10092614580859652No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)