FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17228
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTATCG7314.243092639888554No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTATCT5653.279544926863246No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3862.240538657998607No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2931.7007197585326213No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2431.4104945437659624No Hit
ATTGCGCAATGGCTAGTGGGGGGAGGCATGATGGGGAGCCCATGGTTGTG1200.6965405154399814No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCT1150.6675179939633155No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC1130.6559089853726492No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1000.5804504295333178No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTAT930.5398188994659856No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAT800.46436034362665424No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT700.4063153006733225No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT650.3772927791966566No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT610.3540747620153239No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC480.2786162061759926No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC430.24959368469932666No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCGTATCG410.2379846761086603No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA380.2205711632226608No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC380.2205711632226608No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG370.2147666589273276No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG370.2147666589273276No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG340.19735314604132806No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG340.19735314604132806No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC320.1857441374506617No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA320.1857441374506617No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT310.17993963315532852No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA300.17413512885999535No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTATCG290.1683306245646622No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC250.14511260738332946No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG250.14511260738332946No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG240.1393081030879963No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT230.1335035987926631No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCC230.1335035987926631No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTATCT220.1276990944973299No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT220.1276990944973299No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGC220.1276990944973299No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT220.1276990944973299No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.12189459020199675No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATG210.12189459020199675No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTATCTCG200.11609008590666356No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAT200.11609008590666356No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT200.11609008590666356No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT200.11609008590666356No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT200.11609008590666356No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTAGCG200.11609008590666356No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTATGGCCTTATCGCG190.1102855816113304No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC190.1102855816113304No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG190.1102855816113304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)