FastQCFastQC Report
Tue 7 May 2024
EGAF00007823202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17228
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTA14068.16113303923845No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3912.269561179475273No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3101.7993963315532855No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1851.073833294636638No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.8996981657766427No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACA1210.7023450197353146No Hit
CTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGG1210.7023450197353146No Hit
CTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTC930.5398188994659856No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTG870.5049918736939866No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAA790.4585558393313211No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.4411423264453216No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG560.325052240538658No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.325052240538658No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC550.3192477362433248No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACTGTGTA510.2960297190619921No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA490.2844207104713258No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC470.2728117018806594No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC430.24959368469932666No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC410.2379846761086603No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG380.2205711632226608No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC350.20315765033666125No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGGGTA330.1915486417459949No Hit
ATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGGTGG330.1915486417459949No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGA330.1915486417459949No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG330.1915486417459949No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA310.17993963315532852No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA300.17413512885999535No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA300.17413512885999535No Hit
CATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGGTGGTCGCC300.17413512885999535Illumina Single End PCR Primer 1 (100% over 22bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT290.1683306245646622No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC290.1683306245646622No Hit
CTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGTTCTC280.162526120269329No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTT280.162526120269329No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG270.1567216159739958No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.15091711167866265No Hit
CTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGTTCTCGG250.14511260738332946No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA250.14511260738332946No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCAC240.1393081030879963No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA240.1393081030879963No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC230.1335035987926631No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAA210.12189459020199675No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAG200.11609008590666356No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAATGT200.11609008590666356No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC200.11609008590666356No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA180.10448107731599722No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATTT180.10448107731599722No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGTA180.10448107731599722No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)