FastQCFastQC Report
Tue 7 May 2024
EGAF00007823203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15878
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCG7584.773900995087542No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCT5783.640256959314775No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2231.4044589998740395No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2101.322584708401562No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1510.9510013855649326No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATC1080.6801864214636604No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCAT1000.6298022420959819No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG990.6235042196750221No Hit
ATTGCGCAATGAATGCCCTGGGGGGACAAGCGAATAAGTGCACATGGTGG950.5983121299911828No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC820.5164378385187052No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT800.5038417936767855No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT780.4912457488348659No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT760.4786497039929462No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAG730.45975563673006675No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCG680.4282655246252677No Hit
ATTGCGCAATGGGACTACAGGGGGATCAAGCGACTAAGTGCATATGGTGG680.4282655246252677No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC620.3904773900995087No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT580.3652853004156695No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC580.3652853004156695No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG560.35268925557374986No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG500.31490112104799095No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA460.28970903136415166No Hit
ATTGCGCAATGCTCTCGCGGGTTTAAATAAAAAAATAAAAAAAAAAAAAG410.2582189192593526No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG400.25192089683839275No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC400.25192089683839275No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG370.2330268295755133No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC370.2330268295755133No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT340.21413276231263384No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCT320.20153671747071422No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC290.18264265020783474No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGG290.18264265020783474No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC280.17634462778687493No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT270.1700466053659151No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT250.15745056052399548No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCAT250.15745056052399548No Hit
ATTGCGCAATGGGTAGCAGGGGGGAGACGGAGAACGAAGAGTTTGAAAAA250.15745056052399548No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTA230.14485451568207583No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAG230.14485451568207583No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT230.14485451568207583No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG220.13855649326111602No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT220.13855649326111602No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT210.13225847084015618No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA210.13225847084015618No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA210.13225847084015618No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTG210.13225847084015618No Hit
ATTGCGCAATGGGGGCAGGGGGGAGACGGAGAACGAAGAGTTTGAAAAAC200.12596044841919637No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC200.12596044841919637No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT200.12596044841919637No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCAGCG200.12596044841919637No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCGCG190.11966242599823655No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.11966242599823655No Hit
ATTGCGCAATGCGAAGGTGGGGAAGGCAGACCCGGAGAACTGAAACATCT190.11966242599823655No Hit
ATTGCGCAATGACGATCTTGGGGGAAACGGAGAGAAGAACAGGGTCGAGC190.11966242599823655No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC190.11966242599823655No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG180.11336440357727674No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCTCG180.11336440357727674No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG180.11336440357727674No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT170.10706638115631692No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)