FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12669
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCG5234.128186912937091No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCT4023.17309969216197No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2932.3127318651827293No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2071.633909542978925No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2021.5944431288973084No Hit
ATTGCGCAATGTATGTATCGGGGGGAGGCAGCAGTGGGGAATATTGCACA1301.0261267661220301No Hit
ATTGCGCAATGGGAGAAGGGGGGCCTCATGATGTGAGCACTCTTTGCGGT950.7498618675507144No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTC910.7182887362854211No Hit
ATTGCGCAATGGAGTAGTCGGGGTGTGATCGGGGAGGGTCTGGAAACGGA840.6630357565711579No Hit
ATTGCGCAATGTCAGCCGCGGGAGAAGCGCCAGCTCCACCGTCCTCTGAC810.639355908122188No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGAT690.5446365143263082No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC670.5288499486936618No Hit
ATTGCGCAATGGAGAATGTGGGGTAGAGGGTGAAAATCCCGTACTTGCCA660.5209566658773384No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCG600.4735969689793985No Hit
ATTGCGCAATGCATATACAGGGGTAGAGCGTCCAGCGAATACCCCCGGGG560.44202383771410525No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG550.434130554897782No Hit
ATTGCGCAATGAAATGGCCGGGGGGTTGTAAACCGCTTTCGCCTGTGACG550.434130554897782No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT510.4025574236324887No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC500.39466414081616547No Hit
ATTGCGCAATGGCACAGTTGGGATGGTACAACAGTGAAAGACGGTCTTGC490.3867708579998421No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT400.31573131265293236No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT400.31573131265293236No Hit
ATTGCGCAATGACCTAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA360.28415818138763915No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA340.2683716157549925No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCG330.2604783329386692No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT310.2446917673060226No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG300.23679848448969926No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG260.20522535322440605No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA260.20522535322440605No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG260.20522535322440605No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA250.19733207040808273No Hit
ATTGCGCAATGCAGGCGACGGGGCGCGATTGGCGCAGCGGTAGCGAAACA250.19733207040808273No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC240.18943878759175942No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG240.18943878759175942No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGAGCG240.18943878759175942No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCG230.1815455047754361No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC230.1815455047754361No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC190.14997237351014286No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC180.14207909069381958No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCGCG180.14207909069381958No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT170.13418580787749626No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCT170.13418580787749626No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG170.13418580787749626No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATGG160.12629252506117294No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT150.11839924224484963No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGG140.11050595942852631No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCTCG140.11050595942852631No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC140.11050595942852631No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGAGCT140.11050595942852631No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130.10261267661220302No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG130.10261267661220302No Hit
ATTGCGCAATGTATGCTGCTGCTGTCTCTTATACACATCTCCGAGCCCAC130.10261267661220302No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT130.10261267661220302No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT130.10261267661220302No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT130.10261267661220302No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)