FastQCFastQC Report
Tue 7 May 2024
EGAF00007823215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16694
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTATCG7654.582484725050916No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTATCT5383.2227147478135856No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2931.7551216005750567No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2101.2579369833473104No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1741.0422906433449144No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGC1640.9823888822331376No Hit
ATTGCGCAATGATTCCGAGGGGAGCTCTCGAACCAGGCTGCTTCAGCCGT1570.940457649454894No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTAT920.5510962022283455No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG790.4732239127830358No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG770.4612435605606805No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC670.4013417994489038No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC640.3833712711153708No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT520.31148915778123876No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT520.31148915778123876No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT440.2635677488918174No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTATCG430.25757757278063975No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA360.21564634000239605No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC340.20366598778004072No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTCTCG330.19767581166886306No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTAGCG300.17970528333533006No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG300.17970528333533006No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA290.1737151072241524No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTATCT280.1677249311129747No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA270.16173475500179704No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC270.16173475500179704No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT260.15574457889061938No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG260.15574457889061938No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCGATCG260.15574457889061938No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG260.15574457889061938No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC250.14975440277944171No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACGCTATCGCG240.14376422666826405No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG240.14376422666826405No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG230.13777405055708639No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT230.13777405055708639No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT230.13777405055708639No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG230.13777405055708639No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC220.1317838744459087No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGGACG220.1317838744459087No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGG210.12579369833473103No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG210.12579369833473103No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG200.11980352222355337No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.11980352222355337No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC190.1138133461123757No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT190.1138133461123757No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)