FastQCFastQC Report
Tue 7 May 2024
EGAF00007823217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13149
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCG6364.836869723933379No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCT5123.893832230587877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2121.612289907977793No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1401.0647197505513728No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1331.011483763023804No Hit
ATTGCGCAATGAACAGGCCGGGGGTGGGTAGAATTCCACGTGTAGCAGTG1300.9886683397977032No Hit
ATTGCGCATTGAAGAGGGCGGGAGAACTCACTGAGGATGAGGTGGAACGT1010.7681192486120617No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACT940.7148832610844931No Hit
ATTGCGCAATGGGACTTCCGGGGATGTCATCAAACATCAGGATGACACAC860.6540421324815576No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG840.6388318503308237No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAAT760.577990721727888No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACG640.4867290288234846No Hit
ATTGCGCAATGAGGGCCTTGGGCTTTGATGTGAAAAAGGCTGATGTGTTG630.4791238877481177No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC600.4563084645220169No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT470.35744163054224654No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT470.35744163054224654No Hit
ATTGCGCAATGAGAGGTAGGGGGGTGAAGAAAGGTAAAAGGAATAAGAAT400.30420564301467795No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCG390.296600501939311No Hit
ATTGCGCAATGTCGACGGGGGGGCCGGGAGGGATGGCTCATGCCTGTAAT390.296600501939311No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT360.2737850787132101No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC310.23575937333637542No Hit
ATTGCGCAATGCACGGCCGGGGCGAGGCAGAGATGCGTATCTGATGGGAA290.2205490911856415No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC280.21294395011027456No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC270.20533880903490762No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG260.19773366795954064No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA250.1901285268841737No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG250.1901285268841737No Hit
ATTGCGCAATGATTCTGGGGGGGACAGAGGAAGAATGCTTTTGAAAACAG240.18252338580880675No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATGG240.18252338580880675No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC230.1749182447334398No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT200.15210282150733898No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT190.144497680431972No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC180.13689253935660506No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA180.13689253935660506No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG170.12928739828123811No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAAGCG170.12928739828123811No Hit
ATTGCGCAATGTAATCCACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAG170.12928739828123811No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG160.12168225720587116No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCTCG160.12168225720587116No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG150.11407711613050422No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT150.11407711613050422No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT150.11407711613050422No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCGCG150.11407711613050422No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA140.10647197505513728No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACG140.10647197505513728No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCT140.10647197505513728No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG140.10647197505513728No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC140.10647197505513728No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG140.10647197505513728No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)