FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5642
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3596.362991846862815No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1813.208082240340305No Hit
ATTGCGCAATGCGTCATAAGGGTATGGCCTACCCATTCCAACTTGGTTTA1512.6763559021623538No Hit
ATTGCGCAATGCCTTGAACGGGCGCTGGCGGCGTGCTTAACACATGCAAG791.4002126905352712No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATTAGGAGATCG520.9216589861751152No Hit
ATTGCGCAATGCAGGGGCAGGGAACCATCCACTTCGAGCAGAAGGCAAGC500.8862105636299186No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATTAGGAGATCT490.8684863523573202No Hit
ATTGCGCAATGGCCTTGCTGGGATTTGATGTTAACTCTGTAAGCCAAAGT420.7444168734491315No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG290.5140021269053527No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.4608294930875576No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC230.40765685926976253No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG230.40765685926976253No Hit
ATTGCGCAATGGGGGGAACGGGTGTCCTGCAACTGCTGGAGAGCCTCAAA210.37220843672456577No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAT180.31903580290677064No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATTAGGA170.30131159163417226No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATTCGGAGATCG160.28358738036157394No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATTAGGAGAT130.2304147465437788No Hit
ATTGCGCAATGGGTAGGGGGGGAGAGAGGATATGGTAAAGGAAGAAGACG120.21269053527118043No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT110.19496632399858208No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC110.19496632399858208No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCATTAGGAGATCG100.1772421127259837No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATT100.1772421127259837No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA100.1772421127259837No Hit
ATTGCGCAATGGGCGTAGAGGGAAGTTTCCAAGGGCGCATGGTGGATGCC100.1772421127259837No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA90.15951790145338532No Hit
ATTGCGCAATCTGTCTCTTATACACCGGTCTCTTATACACATCTCCGAGC90.15951790145338532No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT80.14179369018078697No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT80.14179369018078697No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC80.14179369018078697No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT80.14179369018078697No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA80.14179369018078697No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70.12406947890818859No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG60.10634526763559021No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGTAGGAGATCT60.10634526763559021No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT60.10634526763559021No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCATTAGGAGATCT60.10634526763559021No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT60.10634526763559021No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)