FastQCFastQC Report
Tue 7 May 2024
EGAF00007823221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19799
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATATCG8474.27799383807263No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATATCT5872.964796201828375No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1670.8434769432799636No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.8333754230011616No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1340.6768018586797313No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1330.6717510985403303No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTA1190.6010404565887165No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC1070.5404313349159048No Hit
ATTGCGCAATGAGGTTTACGGGGACAGAAATTATCAGAAGCGAAGCGTGA1070.5404313349159048No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATAT960.484872973382494No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC920.46466993282489016No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT880.44446689226728625No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT760.38385777059447446No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT760.38385777059447446No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC710.35860396989746957No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATATCG530.26769028738825196No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC490.24748724683064804No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA480.242436486691247No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC450.22728420627304408No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT450.22728420627304408No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC400.2020304055760392No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG350.1767766048790343No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG350.1767766048790343No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA340.17172584473963332No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC320.16162432446083136No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATATCGCG310.15657356432143038No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG310.15657356432143038No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT280.14142128390322745No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG280.14142128390322745No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC270.13637052376382644No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT260.13131976362442546No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATATCT240.1212182433456235No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCT230.11616748320622254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC220.11111672306682156No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATAGCG220.11111672306682156No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT210.10606596292742057No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG210.10606596292742057No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA210.10606596292742057No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC210.10606596292742057No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT200.1010152027880196No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT200.1010152027880196No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTCTATCG200.1010152027880196No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCA200.1010152027880196No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)