FastQCFastQC Report
Tue 7 May 2024
EGAF00007823225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16442
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCG7574.604062766086851No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCT5723.478895511494952No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2091.2711348984308477No Hit
ATTGCGCAATGAGCTCCCCGGGGGGAGAAAGCAGGGGACCTTCGGGCCTT1871.137331224911811No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1871.137331224911811No Hit
ATTGCGCAATGACATGGGTGGGAACATAAATCTGTGAACTTGAAAATTTA1500.9122977739934315No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1440.8758058630336941No Hit
ATTGCGCAATGCCGCACTTGGGCAGTCACACTGGAACTGAGACACGGTCC1310.7967400559542634No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGAT1040.6325264566354458No Hit
ATTGCGCAATGTATCCCTCGGGGGGAAAGGATGTGGAGTTGCGTAGACAA1040.6325264566354458No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCT890.5412966792361027No Hit
ATTGCGCAATGTCTGCCTGGGGGGATTTTGTTGTAATTGTTTAATCTTAG870.5291327089161902No Hit
ATTGCGCAATGCAAGCCACGGGATGTGTGGTTCAAGTGATGGGGTGACAC820.49872278311640916No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG780.47439484247658437No Hit
ATTGCGCAATGGCCCTGGGGGGATTTTGTTGTAATTGTTTAATCTTAGCA720.43790293151684706No Hit
ATTGCGCAATGGATCCAGCGGGGGGAAAGGATGTGGAGTTGCGTAGACAA630.3831650650772412No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC620.377083079917285No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC600.3649191095973726No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT560.34059116895754776No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT540.3284271986376353No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC540.3284271986376353No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT500.30409925799781046No Hit
ATTGCGCAATGCGCTGACTGGGGACGGCGGTGTTTGGCACCTCGATGTCG490.2980172728378543No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCG460.27977131735798566No Hit
CTCCTTGATGATGCATGGTCGTAACAACGGCAAGAAGCTCATGACTGTGC420.2554433767181608No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA400.2432794063982484No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGAGCG360.21895146575842353No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA360.21895146575842353No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT320.1946235251185987No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC320.1946235251185987No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA300.1824595547986863No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC300.1824595547986863No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG290.17637756963873008No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGAGCT260.15813161415886146No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT250.15204962899890523No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCG250.15204962899890523No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG240.14596764383894903No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG230.13988565867899283No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT220.13380367351903663No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC220.13380367351903663No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC220.13380367351903663No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG220.13380367351903663No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT210.1277216883590804No Hit
ATTGCGCAATGAATTTAGGGGGGATTGTAAAGCACTTTAAGGTGGGAGGA200.1216397031991242No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGG200.1216397031991242No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG190.11555771803916798No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGCTGTCTCTTA190.11555771803916798No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC180.10947573287921177No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGC180.10947573287921177No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCGGATCG180.10947573287921177No Hit
ATTGCGCAATGTACAACCGGGGGGTAGTGCCTCGTAACTAACTCTCGGGG170.10339374771925557No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC170.10339374771925557No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG170.10339374771925557No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCTCG170.10339374771925557No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCT170.10339374771925557No Hit
ATTGCGCAATGCACAACACGGGAAACCTCACCCGGCCCGGACACGGACAG170.10339374771925557No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)