FastQCFastQC Report
Tue 7 May 2024
EGAF00007823226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16442
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTA14178.618172971657948No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2351.4292665125897093No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1911.1616591655516362No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.8332319669140007No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.7359202043547014No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACA1040.6325264566354458No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTG970.5899525605157523No Hit
CTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTC950.57778859019584No Hit
CTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGG950.57778859019584No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC610.3710010947573288No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAA580.35275513927746016No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC550.33450918379759154No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA510.3101812431577667No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC490.2980172728378543No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC480.29193528767789806No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG420.2554433767181608No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC410.24936139155820458No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG390.23719742123829218No Hit
GGATTCCCACCCGTCACACGCTACTCATGCCTGCATTCTCACTCGCATCA390.23719742123829218No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG340.20678749543851113No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA330.20070551027855493No Hit
GAGTTAGCCGGTGCTTATTCTGTCGGTAACGTCAAAACAGTAAAGTATTA330.20070551027855493No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.20070551027855493No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA320.1946235251185987No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACTGTGTA320.1946235251185987No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA300.1824595547986863No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.17637756963873008No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA280.17029558447877388No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGA280.17029558447877388No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGGGTA270.16421359931881765No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAA250.15204962899890523No Hit
GTATTAATTCACTGCCCTTCCTCCCAACTTAAAGTGCTTTACAATCCGAA250.15204962899890523No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTT250.15204962899890523No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC250.15204962899890523No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAG240.14596764383894903No Hit
CATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGGTGGTCGCC240.14596764383894903Illumina Single End PCR Primer 1 (100% over 22bp)
CACACGCTACTCATGCCTGCATTCTCACTCGCATCAACTCCACCACACAG210.1277216883590804No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG210.1277216883590804No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC210.1277216883590804No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA210.1277216883590804No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGA210.1277216883590804No Hit
ATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGATCTCGGTGG210.1277216883590804No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG210.1277216883590804No Hit
GTAATAAACATGGCTTTAATATTAAACTTTTTCCTTATTATCCAAAGTCA210.1277216883590804No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.1277216883590804No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACAGAAGCAC200.1216397031991242No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC200.1216397031991242No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA200.1216397031991242No Hit
CTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGTTCTCGG200.1216397031991242No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA200.1216397031991242No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA180.10947573287921177No Hit
CTCTTATACACATCTGACGCTGCCGACGACAGAAGCACAGTGTAGTTCTC180.10947573287921177No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC180.10947573287921177No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT180.10947573287921177No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCC170.10339374771925557No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA170.10339374771925557No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)