FastQCFastQC Report
Tue 7 May 2024
EGAF00007823229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13115
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCG4453.393061380099123No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCT3492.661075104841784No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2181.6622188333968737No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1371.0446054136484941No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1160.8844834159359513No Hit
ATTGCGCAATGTGAACCACGGGATAAGCGCACACGGTGGATGCCTTGGCG940.7167365611894777No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG740.5642394205108654No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCT650.49561570720548986No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC640.4879908501715593No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCAT600.4574914220358368No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT480.3659931376286695No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA430.32786885245901637No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC420.32024399542508575No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT390.29736942432329394No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT360.2744948532215021No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC300.2287457110179184No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGTCTCTCATCG300.2287457110179184No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA270.20587113991612657No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA250.19062142584826536No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGTTCTCTCATCG230.17537171178040412No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC230.17537171178040412No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG230.17537171178040412No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT220.16774685474647352No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG220.16774685474647352No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG210.16012199771254287No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGTTCTCTCATCT200.15249714067861228No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCG190.14487228364468166No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG190.14487228364468166No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT180.13724742661075104No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG180.13724742661075104No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.12962256957682045No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTC170.12962256957682045No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATGTCTCATCG160.12199771254288982No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAT160.12199771254288982No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGTCTCTCATCT160.12199771254288982No Hit
ATTGCGCAATGTTACCTTCGGGACGGTTAAGTTCGCTTACAAGGTGAACC150.1143728555089592No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC150.1143728555089592No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG150.1143728555089592No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCTCG150.1143728555089592No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG140.1067479984750286No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT140.1067479984750286No Hit
ATTGCGCAATCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA140.1067479984750286No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT140.1067479984750286No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)