FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948
Sequences flagged as poor quality0
Sequence length151
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT32516.68377823408624No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1135.800821355236139No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC402.0533880903490758No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGGTGGTCGCC130.6673511293634496Illumina RNA PCR Primer (95% over 23bp)
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGTGGGGGGGGGGG80.41067761806981523No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGGGGGGGGC60.3080082135523614No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGGGGGGGGG60.3080082135523614No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTCGGGGGGGGGG60.3080082135523614No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGGGGGGGGGGGG50.25667351129363447No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGG50.25667351129363447No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGTCGG40.20533880903490762No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.20533880903490762No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGTCGGGGGGGGGG40.20533880903490762No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA40.20533880903490762No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTGGTGTGGGGGGGGGGG40.20533880903490762No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGTGGG40.20533880903490762No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTCGGGGGGGGGG40.20533880903490762No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTG40.20533880903490762No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTCGG40.20533880903490762No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGT40.20533880903490762No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTC30.1540041067761807No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGCGGGGGGGGGG30.1540041067761807No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGGGGGGGGGGGGG30.1540041067761807No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGGGGGGGGGG30.1540041067761807No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGATGTG30.1540041067761807No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTGTGGGGGGGGGGG30.1540041067761807No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGGGGGGGGGGGGG30.1540041067761807No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGAGTTCTCGG30.1540041067761807No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTGTCGGGGGGGGGG30.1540041067761807No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGGGGGGGGGGGGG30.1540041067761807No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGAGTTGTG30.1540041067761807No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGGGGG30.1540041067761807No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTCTCGG30.1540041067761807No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGA30.1540041067761807No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGG30.1540041067761807No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGTGGGGGGG30.1540041067761807No Hit
GGTCCCGACTAACCCTGGGAGGACGAGCCTTCCCCAGGAAACCTTAGTCA30.1540041067761807No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAATGT20.10266940451745381No Hit
CTCCCGCCTCAGCCTCCTGAGTAGCTAGGACCACAGGCGCCTGCCACCAC20.10266940451745381No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGTCGGGGG20.10266940451745381No Hit
ACTTTCCAGTTACGACTACGGTTTATTGCTAGAAAAGTCATTACCTTTCC20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGCGGGGGGGGGC20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGAGATGTGGGGGGGGGGG20.10266940451745381No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGATGTGGGGGG20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTGGTGGGGGGGGGGG20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGGGGGGGGGC20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGATGTGGGTGGGGGGG20.10266940451745381No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTGGTGTCGGGGGGGGGC20.10266940451745381No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCAAAAAATT20.10266940451745381No Hit
ATCTGTCTCTTATACACATCTGACGCTGTCGACGATGACGGTGAGGTGTA20.10266940451745381No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTGGTTGTCGGGGG20.10266940451745381No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTTGGTGTGGG20.10266940451745381No Hit
GCAGCAGACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATCT20.10266940451745381No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATAAACATCTGACGCT20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGATGTGGGGGGGGGGG20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTGGGTGGCGGGGGGGGGG20.10266940451745381No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTCTGGGGGG20.10266940451745381No Hit
TGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTG20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTCTGGGTGGGGGGG20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTGGTGTCGGTGGGGGGG20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGGCGGGGGGGGGG20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGAGTTGTGGGGGGGGGGC20.10266940451745381No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTGTG20.10266940451745381No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTG20.10266940451745381No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTGTACACATCTGAC20.10266940451745381No Hit
GATACTTATTTTCTTTAGGGTGCTGATCATATCATCCAATGCCTTGCGGT20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTTTTGTCGGGGGGGGGG20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGAGGTCTCGGGGGGGGGG20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGTCGGGGGGGGGG20.10266940451745381No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTT20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTTTTATAGGTGGGGGGG20.10266940451745381No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTA20.10266940451745381No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTTGGTGTG20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGGGGGGGGGGGGC20.10266940451745381No Hit
GATCTGGGCTCACTGCAACCTCCACCTCCCGGGTCCAAGCGACTCTCCCG20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGATGGGGGGGGGGGGG20.10266940451745381No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGAGTGCTCGG20.10266940451745381No Hit
GTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10266940451745381No Hit
ATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACG20.10266940451745381No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTAAACACATCTGACGCT20.10266940451745381No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGGTGG20.10266940451745381No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGA20.10266940451745381No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTCGGGGG20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGGCGGGGGGGGGG20.10266940451745381No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAAT20.10266940451745381No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTTGGTGTCGGGGG20.10266940451745381No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTGGGTGTG20.10266940451745381No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTATATCTCGGTGGTCGCC20.10266940451745381Illumina Single End PCR Primer 1 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGG20.10266940451745381No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTGGGGGG20.10266940451745381No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATTTA20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTGTGGGTGGGGGGG20.10266940451745381No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGGGGGGG20.10266940451745381No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGGGGGTGGGG20.10266940451745381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)