FastQCFastQC Report
Tue 7 May 2024
EGAF00007823233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17660
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAATCG6063.4314835787089466No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAATCT4792.712344280860702No Hit
ATTGCGCAATGGCGGATACGGGGGAGTTGTATAAACTTTCCTGGAGACTG3221.8233295583238958No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2891.636466591166478No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2771.5685164212910532No Hit
ATTGCGCAATGCACCACTCGGGCAAGCCAGTAGCACAGCTCTACTGTTTA2121.200453001132503No Hit
ATTGCGCAATGTTGGTACTGGGGCTTTCCTTTCTGAGCCCCGGCGGTGCG1740.9852774631936579No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1720.9739524348810872No Hit
ATTGCGCAATGCCCCTGGCGGGCGGTCGCAAGATTAAAACTCAAAGGAAT1590.9003397508493771No Hit
ATTGCGCAATGACGACAGCGGGGGGTATTGGTCTTGACTACGGTTTGTGT1460.8267270668176671No Hit
ATTGCGCAATGCCAGGGGGGGGGAGACAGAGGAACAGGAGCAACACGAAG1350.7644394110985278No Hit
ATTGCGCAATGCGGATTGGGGGAAGTTCAACACGGTGGACATGCGCATCA1180.6681766704416762No Hit
ATTGCGCAATGGCGTAGCGGGGGGTATTGGTCTTGACTACGGTTTGTGTA940.5322763306908267No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGT920.5209513023782559No Hit
ATTGCGCAATGCAATAGGGGGGTCCGTGCAAGTGCTAACATATCTCCAGG900.5096262740656852No Hit
ATTGCGCAATGGTCGGCTCGGGGTGTCCGCGTTGTAATCTCGAGACGTGT760.4303510758776897No Hit
ATTGCGCAATGCGAGCGCCGGGTCCGAATGGGGGAACCCAGCATGAGTTA660.3737259343148358No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG640.362400906002265No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAAT610.3454133635334088No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC560.3171007927519819No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC530.30011325028312574No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAC480.2718006795016987No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA460.26047565118912797No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT400.22650056625141565No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT370.20951302378255945No Hit
ATTGCGCAATGCACTTCCGGGGGCATCGCCTGGTAGCTATGTTCGGAAGA370.20951302378255945No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAC350.19818799546998866No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA340.19252548131370328No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC290.16421291053227632No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT290.16421291053227632No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA260.14722536806342015No Hit
ATTGCGCAATGGGGGGGGGGGAGACAGAGGAACAGGAGCAACACGAAGAG250.14156285390713477No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAATCG250.14156285390713477No Hit
ATAACATGCTGATCACCCTCCTCCCAAAAAGCAAGAGCTTGTTTATGGCT240.13590033975084936No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG230.13023782559456398No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT220.1245753114382786No Hit
ATTGCGCAATGGGGGGGGGGGGAGACAGAGGAACAGGAGCAACACGAAGA220.1245753114382786No Hit
ATTGCGCAATGCTGTCCACGGGGTAAAGGAGGTGGGTAGATGGGGAAAAA210.11891279728199322No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAATCGCG200.11325028312570783No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC190.10758776896942243No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC190.10758776896942243No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG190.10758776896942243No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC180.10192525481313704No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG180.10192525481313704No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG180.10192525481313704No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)