FastQCFastQC Report
Tue 7 May 2024
EGAF00007823234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17660
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTA11926.749716874292186No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3431.9422423556058892No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2281.291053227633069No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.8380520951302378No Hit
CTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGG1290.7304643261608154No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.6964892412231031No Hit
CTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTC990.5605889014722537No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCT970.549263873159683No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGTCGCC680.38505096262740657Illumina Single End PCR Primer 1 (95% over 22bp)
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTG620.3510758776896942No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.3171007927519819No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT520.2944507361268403No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAAT510.2887882219705549No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG480.2718006795016987No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC440.2491506228765572No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG420.23782559456398644No Hit
ATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGG400.22650056625141565No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAAT390.22083805209513024No Hit
GGATAAAGAGCATCTTATCTTTGTGGCTCAGTTTGTCAAATCCTCTTTTA330.1868629671574179No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC330.1868629671574179No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.1868629671574179No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA320.1812004530011325No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC310.1755379388448471No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.1755379388448471No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA300.16987542468856173No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG270.15288788221970553No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG250.14156285390713477No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGA250.14156285390713477No Hit
GTTTTATACTTAGTATACTAAATTTTGTCTTATATATTTGGAATCCAATC220.1245753114382786No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGGCGCC210.11891279728199322Illumina RNA PCR Primer (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA210.11891279728199322No Hit
GTCCATGGTTTCATAGATATTTTTCTCGTTTAACTGAAATAGGTTTAATT210.11891279728199322No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC210.11891279728199322No Hit
TCCTTAGAGTGCCCAACTGAATGATGGCAACTAGGGATGAGGGTTGCGCT200.11325028312570783No Hit
CTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGTTCTCGG200.11325028312570783No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA190.10758776896942243No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG190.10758776896942243No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC180.10192525481313704No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)