FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007823242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8986
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTA6527.255731137324728No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1912.1255286000445137No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1391.5468506565768974No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.9347874471399956No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.9236590251502338No Hit
CTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGG750.8346316492321388No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCT650.7233474293345203No Hit
CTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTC560.6231916314266637No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAAT500.5564210994880926No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGTCGCC500.5564210994880926Illumina Single End PCR Primer 1 (95% over 22bp)
ATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGG490.5452926774983307No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTG450.5007789895392833No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT450.5007789895392833No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG370.41175161362118856No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG340.37836634765190297No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG270.30046739372357No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG250.2782105497440463No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.2782105497440463No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC250.2782105497440463No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC230.2559537057645226No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.2559537057645226No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC190.21144001780547517No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA190.21144001780547517No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGGCGCC170.18918317382595148Illumina RNA PCR Primer (95% over 21bp)
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAAT170.18918317382595148No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA170.18918317382595148No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC170.18918317382595148No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGA170.18918317382595148No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC160.17805475183618963No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA160.17805475183618963No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGTGGCC150.16692632984642777Illumina RNA PCR Primer (95% over 21bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT140.15579790785666592No Hit
CTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGTTCTCGG140.15579790785666592No Hit
GTACCACACAGGTCATTTCACGTACGGGGCTATCACCCGCTATGGCCAGC130.14466948586690406No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA120.13354106387714224No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGTCGGC120.13354106387714224Illumina RNA PCR Primer (95% over 21bp)
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGGGGTCGCC120.13354106387714224Illumina RNA PCR Primer (95% over 21bp)
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.13354106387714224No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC120.13354106387714224No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA120.13354106387714224No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA110.12241264188738038No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA110.12241264188738038No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATC110.12241264188738038No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA110.12241264188738038No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG110.12241264188738038No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC110.12241264188738038No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.12241264188738038No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAT100.11128421989761851No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGT90.10015579790785667No Hit
GCTGTTCCGTTATGTCTTGTGCTATCACTAACAGGCTTCTCCGATTTCGC90.10015579790785667No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATC90.10015579790785667No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA90.10015579790785667No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG90.10015579790785667No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAA90.10015579790785667No Hit
CAGCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG90.10015579790785667No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA90.10015579790785667No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)