FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14909
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCG6224.171976658394259No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCT5513.695754242403917No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2491.6701321349520424No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1440.9658595479240728No Hit
ATTGCGCAATGGGTGGTCGGGGGGTGGTGGTCTGGATCCGCTTACCGACG1320.8853712522637333No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1020.6841505131128849No Hit
ATTGCGCAATGAGTAGGTAGGGGAGTGGACGGAGATCCTCAAGTATTTCC850.5701254275940707No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG800.536588637735596No Hit
ATTGCGCAATGGATGACGTGGGTCCTCTTCAACGACATCATGCCAGAGGG770.516466563820511No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACT750.5030518478771212No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACG600.40244147830169696No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC600.40244147830169696No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC570.3823194043866121No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAAT550.3689046884432222No Hit
ATTGCGCAATGGGCGCATGGGGATGCTTGTCTCAAAGATTAAGCCATGCA540.36219733047152725No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT490.3286605406130525No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT420.28170903481118786No Hit
ATTGCGCAATGATGTTGAAGGGGGTTCCAGGAAATAGCCCCTCCATTATA420.28170903481118786No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA380.25487960292440803No Hit
ATTGCGCAATGTTTGTGCCGGGGGCGCAGGCCATCATAAAGCAGTACCCG380.25487960292440803No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT370.24817224495271312No Hit
ATTGCGCAATGCTGATTGTGGGGAAGCGCCGGCTAACTACGTGCCAGCAG360.2414648869810182No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC310.20792809712254345No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC300.20122073915084848No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACG300.20122073915084848No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCG300.20122073915084848No Hit
ATTGCGCAATGTCAATATCGGGCAACGTAAACGAACCAGGAGACGTCGGC290.19451338117915354No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG280.1878060232074586No Hit
ATTGCGCAATGACCTAACAGGGGACTGTTGGAAACGATTTGGAAGGTTCG270.18109866523576362No Hit
ATTGCGCAATGGTGAGGGGGGGTCACTCGTTTGCATCAATGAACCGGATT260.17439130726406868No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT260.17439130726406868No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA250.16768394929237374No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC250.16768394929237374No Hit
ATTGCGCAATGACATAACGGGGAAGTTATTAAGGGCGCACGGTGGATGCC240.1609765913206788No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATGG230.15426923334898385No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC220.14756187537728888No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT220.14756187537728888No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAAGCG220.14756187537728888No Hit
ATTGCGCAATGATGGTACCGGGCGCGTCAGGCAACGTTGCACGTTAGCGG210.14085451740559393No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG210.14085451740559393No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT210.14085451740559393No Hit
ATTGCGCAATGCGCATATGGGGCGTTATTAAGGGCGCATGGTGGATGCCT210.14085451740559393No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT200.134147159433899No Hit
ATTGCGCAATGGGCAGCCGGGGGCGCAGGCCATCATAAAGCAGTACCCGC200.134147159433899No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG200.134147159433899No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG190.12743980146220402No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGC180.1207324434905091No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACG170.11402508551881414No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTAC170.11402508551881414No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT160.10731772754711918No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA160.10731772754711918No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150.10061036957542424No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG150.10061036957542424No Hit
ATTGCGCAATGGGGGCACCGGGGTTGGCCAGCGTAGACAATCTAAGAGAT150.10061036957542424No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT150.10061036957542424No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC150.10061036957542424No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTACTAATCGCG150.10061036957542424No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)