FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14909
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTA12908.652491783486486No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1781.193909718961701No Hit
CTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGG1360.9122006841505131No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.8987859682071232No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1270.8518344624052586No Hit
CTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTC970.65061372325441No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.6371990073110202No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCT760.5097592058488161No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG630.42256355221678177No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG620.4158561942450869No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT600.40244147830169696No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTG580.3890267623583071No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG550.3689046884432222No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAAT550.3689046884432222No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC500.33536789858474747No Hit
ATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGG420.28170903481118786No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGTCGCC360.2414648869810182Illumina Single End PCR Primer 1 (95% over 22bp)
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.2414648869810182No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAAT350.23475752900932323No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC300.20122073915084848No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC300.20122073915084848No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC300.20122073915084848No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA290.19451338117915354No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.19451338117915354No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.19451338117915354No Hit
GCAGATGAAGGCCTGTCATTTTCTTCATATAGTGCAACAAGAACTTTAGT280.1878060232074586No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA280.1878060232074586No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT270.18109866523576362No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAA260.17439130726406868No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG250.16768394929237374No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGA230.15426923334898385No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC230.15426923334898385No Hit
GGCCTGTCATTTTCTTCATATAGTGCAACAAGAACTTTAGTGAGAGCATC220.14756187537728888No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATC220.14756187537728888No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA200.134147159433899No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA200.134147159433899No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAT170.11402508551881414No Hit
CTTATACACATCTGACGCTGCCGACGATCAATCTGCTGTGTAGTTCTCGG170.11402508551881414No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAATCTGC170.11402508551881414No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC170.11402508551881414No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGGCGCC160.10731772754711918Illumina RNA PCR Primer (95% over 21bp)
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA160.10731772754711918No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAAT160.10731772754711918No Hit
CATCTGACGCTGCCGACGATCAATCTGCTGTGTAGATCTCGGTGGTGGCC150.10061036957542424Illumina RNA PCR Primer (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA150.10061036957542424No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA150.10061036957542424No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)