FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12974
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCG4173.214120548789887No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCT3002.3123169415754585No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1801.3873901649452751No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1661.2794820410050871No Hit
ATTGCGCAATGAATTACGGGGGGGATGACTAAGGGTTCCTGGGTCAAGTT1280.9865885617388624No Hit
ATTGCGCAATGGAGGGTCAGGGGTCAAGTCATTCTTGTCTAGGAATCAAG1240.9557576691845229No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1070.8247263758285802No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCT820.6320332973639587No Hit
ATTGCGCAATGGGTTCATAGGGATTCGTATCAACAAGATGCTTTCGTGCG760.5857869585324496No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG740.5703715122552797No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCAT620.47787883459226144No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA480.3699707106520734No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC470.3622629875134885No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC460.35455526437490364No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT440.3391398180977339No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT400.30830892554339445No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC380.2928934792662248No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT360.27747803298905505No Hit
ATTGCGCAATGGTCCCCTTGGGGGGGACAAATGCTGAAGAACGATAAGAA330.25435486357330045No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA310.23893941729613072No Hit
ATTGCGCAATGAAATGCCAGGGTCTACGTAAGCAGTTTAATAATAATCCA300.23123169415754588No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC250.19269307846462155No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGTCTCTCATCG250.19269307846462155No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG240.1849853553260367No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC240.1849853553260367No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA220.16956990904886696No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA190.1464467396331124No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTC190.1464467396331124No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG190.1464467396331124No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATGTCTCATCG180.13873901649452752No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT180.13873901649452752No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGTCTCTCATCT180.13873901649452752No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGA170.13103129335594266No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG170.13103129335594266No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGTTCTCTCATCT170.13103129335594266No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA160.1233235702173578No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT160.1233235702173578No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC150.11561584707877294No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGTTCTCTCATCG150.11561584707877294No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT140.10790812394018806No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT140.10790812394018806No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCG130.1002004008016032No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGATCTCTCATCTCG130.1002004008016032No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)