FastQCFastQC Report
Tue 7 May 2024
EGAF00007823269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14504
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAATCT6754.653888582460011No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAATCG4783.2956425813568666No Hit
ATTGCGCAATGAAGGGCTGGGGGGGGCGACCTCTTCCTGCGTTCTCACGG4152.861279646993933No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2992.06150027578599No Hit
ATTGCGCAATGCTCTCCGCGGGAGACCAAGGGTTCCAGTCCAACGTTAAT2922.0132377275234417No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2331.6064533921676778No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1531.054881412024269No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGT880.6067291781577496No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAAT660.4550468836183122No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG630.4343629343629344No Hit
ATTGCGCAATGTAGGGCCAGGGTCGCGACCTCAGATCAGACGTGGCGACC600.4136789851075565No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT560.3861003861003861No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC480.3309431880860452No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT460.31715388858246No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAC450.3102592388306674No Hit
ATTGCGCAATGAACGAGAGAAAAGAAGGAATAAGGGAGAGTATTGGTAAG400.27578599007170435No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT380.26199669056811914No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC310.21373414230557086No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC300.20683949255377826No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG270.18615554329840042No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA270.18615554329840042No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA260.17926089354660782No Hit
ATTGCGCAATGCCGAGGGGGGGTTGATGCTTCTGGGGTGGGTGAGTGTGT260.17926089354660782No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAATCT250.17236624379481522No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAAGCG240.1654715940430226No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG220.1516822945394374No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG220.1516822945394374No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC220.1516822945394374No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAATCG210.14478764478764478No Hit
ATTGCGCAATGTAAGGACGGGGGGAACACTTTTACACTGTTGGTGGAAAC210.14478764478764478No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT200.13789299503585217No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA200.13789299503585217No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAC200.13789299503585217No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG200.13789299503585217No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT190.13099834528405957No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC180.12410369553226695No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.12410369553226695No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGA180.12410369553226695No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT180.12410369553226695No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGGAATCG180.12410369553226695No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAATCTCG170.11720904578047435No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAATCGCG160.11031439602868175No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAAGCT160.11031439602868175No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG150.10341974627688913No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)