FastQCFastQC Report
Tue 7 May 2024
EGAF00007823277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13638
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCT5544.062179205162048No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCG4683.43158820941487No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2411.7671212787798798No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1571.1511951899105441No Hit
ATTGCGCAATGCGAACCCGGGGAACTGAAACATCTAAGTACCCGGAGGAA1320.9678838539375276No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATC1250.9165566798650828No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1160.850564598914797No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCAT680.4986068338466051No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC630.4619445666520018No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG620.4546121132130811No Hit
ATTGCGCAATGACAGAGGGGGGGGAACCGAACACCGGGAACCTGCCTTCC620.4546121132130811No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT590.43261475289631907No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAG580.4252822994573985No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT530.3886200322627952No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT520.38128757882387443No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC470.3446253116292711No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC420.3079630444346678No Hit
ATTGCGCAATGCAAGACCCGGGCTGTGTTGTTTGTAATGTCTAATGAGAT380.2786332306789852No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT380.2786332306789852No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG370.2713007772400645No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCG360.2639683238011439No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC340.24930341692330255No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCT330.2419709634843819No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG310.22730605660654055No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCTCG300.21997360316761988No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA280.20530869628977857No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC270.19797624285085788No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT270.19797624285085788No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGG260.19064378941193721No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCAT250.18331133597301658No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCAGCG250.18331133597301658No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAG220.16131397565625458No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA210.1539815222173339No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG210.1539815222173339No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG210.1539815222173339No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCAGCT210.1539815222173339No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA200.14664906877841327No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG200.14664906877841327No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT200.14664906877841327No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT190.1393166153394926No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG170.12465170846165127No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT170.12465170846165127No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTA160.11731925502273059No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC160.11731925502273059No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT160.11731925502273059No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT160.11731925502273059No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCGCG150.10998680158380994No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.10998680158380994No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC150.10998680158380994No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT150.10998680158380994No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG150.10998680158380994No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTG140.10265434814488929No Hit
ATTGCGCAATGTAGACTTCGGGCAGTACCGGGAGAATAAGCTCCGGCTAA140.10265434814488929No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT140.10265434814488929No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC140.10265434814488929No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTG140.10265434814488929No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)