FastQCFastQC Report
Tue 7 May 2024
EGAF00007823279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9446
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATCT3673.8852424306584794No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATCG2812.9748041499047218No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1181.2492060131272495No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1141.2068600465805632No Hit
ATTGCGCAATGTCATCGCAGGGGGGAACGGCCCCCTCGCGGGGGCCTTCC870.9210247723904299No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC700.7410544145670125No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGC610.645775989836968No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGAT370.3917001905568495No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG370.3917001905568495No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGT350.37052720728350624No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT320.33876773237349145No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC310.3281812407368198No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT280.29642176582680496No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT210.22231632437010374No Hit
ATTGCGCAATGGGACAGGGGGGGAAAGGGCGCAGGGTTTGAAACATGGCG210.22231632437010374No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGAGCG210.22231632437010374No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA200.21172983273343213No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC200.21172983273343213No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC190.20114334109676055No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGAGCT180.1905568494600889No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGT170.1799703578234173No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCAGGAGACTCGTGAAGCGATCT170.1799703578234173No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG160.16938386618674572No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA160.16938386618674572No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC160.16938386618674572No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTG150.15879737455007412No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATCTCG140.14821088291340248No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGT140.14821088291340248No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT140.14821088291340248No Hit
ATTGCGCAATGAGACAGAGGGGGAAAGGGCGCAGGGTTTGAAACATGGCG130.13762439127673087No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG120.1270378996400593No Hit
ATTGCGCAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGT120.1270378996400593No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG120.1270378996400593No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC110.11645140800338769No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT110.11645140800338769No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT110.11645140800338769No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG110.11645140800338769No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT100.10586491636671606No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA100.10586491636671606No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT100.10586491636671606No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAG100.10586491636671606No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGTGAAGCGATGT100.10586491636671606No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCAGGAGACTCGTGAAGCGATCG100.10586491636671606No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)