FastQCFastQC Report
Tue 7 May 2024
EGAF00007823281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9702
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCT3833.9476396619253764No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCG3053.1436817151102865No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1421.463615749330035No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1321.3605442176870748No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC960.9894867037724181No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC460.47412904555761703No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT450.463821892393321No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGAT440.45351473922902497No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTC400.4122861265718408No Hit
ATTGCGCAATGAGTGGCCCGGGGGCTCATTAAATCAGTTATCGTTTATTT400.4122861265718408No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCG380.3916718202432488No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG360.3710575139146568No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC340.35044320758606473No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGAGCG300.30921459492888065No Hit
ATTGCGCAATGTCCTTGACGGGGTGCAAAAAAGACAGCAGAAGAATAACA270.27829313543599254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT260.26798598227169657No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGAGCT260.26798598227169657No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCG250.25767882910740053No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC250.25767882910740053No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCG240.24737167594310452No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT200.2061430632859204No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT200.2061430632859204No Hit
ATTGCGCAATGGCCGTGGTGGGGCGGCGGACTAGGAGCACCACCTGTGCG170.17522160379303237No Hit
ATTGCGCAATGGGTGTGACGGGGGCGCCTGGCTCACGAGGTTGGCTGGAA170.17522160379303237No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC150.15460729746444032No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATGG140.1443001443001443No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCT140.1443001443001443No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC140.1443001443001443No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA140.1443001443001443No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT130.13399299113584828No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT120.12368583797155226No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATT120.12368583797155226No Hit
ATTGCGCAATGATCCGTGAGGGGAACTAAAAGTCCGCTCGGACTTTTGGC120.12368583797155226No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCGCG120.12368583797155226No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT120.12368583797155226No Hit
ATTGCGCAATGGGGGGAAGGGGATGGGAACTCCAGATGTGCGCATTGACA110.11337868480725624No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC110.11337868480725624No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGAGTCGATCG100.1030715316429602No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGGATTCGATCTCG100.1030715316429602No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.1030715316429602No Hit
ATTGCGCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGG100.1030715316429602No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG100.1030715316429602No Hit
ATTGCGCAAAAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGA100.1030715316429602No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)