FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007823282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9702
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTA7547.771593485879199No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1691.7419088847660276No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1341.3811585240156667No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.8245722531436817No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGG790.8142650999793857No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.7936507936507936No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGGGTA630.6493506493506493No Hit
CTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTC520.5359719645433931No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTG480.49474335188620905No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGG400.4122861265718408No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGT380.3916718202432488No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT370.3813646670789528No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG330.3401360544217687No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC310.3195217480931767No Hit
ATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGG270.27829313543599254No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.24737167594310452No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA230.23706452277880852No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG230.23706452277880852No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGA220.22675736961451248No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGT220.22675736961451248No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG200.2061430632859204No Hit
CATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGGTCGCC200.2061430632859204Illumina Single End PCR Primer 1 (95% over 23bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA190.1958359101216244No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTT190.1958359101216244No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC180.1855287569573284No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCG180.1855287569573284No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.1855287569573284No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.1855287569573284No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA170.17522160379303237No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT150.15460729746444032No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTG150.15460729746444032No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT130.13399299113584828No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC130.13399299113584828No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC130.13399299113584828No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG120.12368583797155226No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT120.12368583797155226No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC120.12368583797155226No Hit
TTTTTTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA110.11337868480725624No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA110.11337868480725624No Hit
CATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTC110.11337868480725624No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAAA100.1030715316429602No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATC100.1030715316429602No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA100.1030715316429602No Hit
CTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGTTCTC100.1030715316429602No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG100.1030715316429602No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC100.1030715316429602No Hit
ATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGGGG100.1030715316429602No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)