FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13545
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTA10747.929125138427465No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2321.7128091546696196No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1621.196013289036545No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGG1190.8785529715762275No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.8785529715762275No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGG1100.8121077888519748No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.6570690291620525No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGGGTA860.6349206349206349No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTG780.5758582502768549No Hit
CTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTC740.546327057954965No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGT650.47988187523071246No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC500.36913990402362495No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG490.3617571059431524No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA450.33222591362126247No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.33222591362126247No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG400.29531192321889993No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG360.26578073089701No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC350.2583979328165375No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.2583979328165375No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG340.25101513473606496No Hit
ATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGG290.21410114433370245No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC280.20671834625322996No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC280.20671834625322996No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT270.19933554817275745No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT250.18456995201181248No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA240.17718715393134No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGA230.16980435585086748No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA220.162421557770395No Hit
CATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGGTCGCC220.162421557770395Illumina Single End PCR Primer 1 (95% over 23bp)
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC220.162421557770395No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC210.15503875968992248No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGTTCTCGG190.14027316352897748No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTT190.14027316352897748No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT180.132890365448505No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTG180.132890365448505No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG180.132890365448505No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA170.12550756736803248No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA170.12550756736803248No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCG160.11812476928755998No Hit
CTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGTTCTC160.11812476928755998No Hit
CTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGGGTAGATCTC150.11074197120708748No Hit
CATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGGTGGCC150.11074197120708748No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA150.11074197120708748No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC140.10335917312661498No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA140.10335917312661498No Hit
GGCTTGGGCAGCGTGTCCGGACCTGTCTCCTGCGCATCGTCGACCTCGGA140.10335917312661498No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGGCTTGGGTGTA140.10335917312661498No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC140.10335917312661498No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAA140.10335917312661498No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)