FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12558
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCATCT5504.3796782927217714No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCATCG3883.089663959229177No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1871.4890906195254021No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1801.433349259436216No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCT1020.8122312470138557No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC930.7405637840420449No Hit
ATTGCGCAATGACGTGGGGGGGCCGGCGGCCGAAGACACTGCGACTCCGG890.7087115782767957No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCAT870.692785475394171No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG670.5335244465679247No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCA650.5175983436853002No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC580.461856983596114No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT560.4459308807134894No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT480.3822264691829909No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT420.33444816053511706No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCATCG400.3185220576524925No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCATCT360.2866698518872432No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG350.2787068004459309No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC310.24685459468068166No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC310.24685459468068166No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG310.24685459468068166No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG310.24685459468068166No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT310.24685459468068166No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT300.23889154323936934No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC280.2229654403567447No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC280.2229654403567447No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG250.19907628603280775No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC240.19111323459149546No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA240.19111323459149546No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC240.19111323459149546No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA220.17518713170887085No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG220.17518713170887085No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT210.16722408026755853No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCAGCG200.15926102882624624No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTG200.15926102882624624No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGC180.1433349259436216No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG180.1433349259436216No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT180.1433349259436216No Hit
ATTGCGCAATGCAAAGCATGGGGAAACCCATCAATTAAAGACCTAGTATT170.13537187450230928No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCAGCT160.12740882306099696No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTG160.12740882306099696No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAA160.12740882306099696No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT160.12740882306099696No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCC150.11944577161968467No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC150.11944577161968467No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT140.11148272017837235No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT140.11148272017837235No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT130.10351966873706005No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAATCCTCATCTCG130.10351966873706005No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC130.10351966873706005No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)