FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13349
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCT6985.228856094089445No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCG5253.9328788673308863No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1801.3484156116563037No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1421.0637500936399729No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAG1341.003820510899693No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1160.8689789497340625No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG850.6367518166154768No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGC750.5618398381901266No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAAT740.5543486403475916No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT600.4494718705521013No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC600.4494718705521013No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT570.42699827702449616No Hit
ATTGCGCAATGAACTACGCGGGAAGGATAACAAGAGAGAAAAACAAAGAA510.3820510899692861No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT460.344595100756611No Hit
ATTGCGCAATGATTCTCGGGGGGTGACATGACTCTTCGCAGGCAGTGGTT460.344595100756611No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT430.32212150722900595No Hit
ATTGCGCAATGACTACTGTGGGCCTTCAGGGAGCTCAATTCTACAAAGGT430.32212150722900595No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCG420.3146303093864709No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCT410.30713911154393586No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC390.2921567158588658No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC380.2846655180163308No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG330.2472095288036557No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC290.21724473743351563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC280.2097535395909806No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA250.18727994606337553No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG240.1797887482208405No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT240.1797887482208405No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC230.1722975503783055No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG230.1722975503783055No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCTCG220.16480635253577047No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAAGCT220.16480635253577047No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA210.15731515469323545No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATCGCG210.15731515469323545No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGC200.14982395685070043No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT200.14982395685070043No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAATGG190.1423327590081654No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGC190.1423327590081654No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGC180.1348415611656304No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC170.12735036332309535No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA170.12735036332309535No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT170.12735036332309535No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC170.12735036332309535No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG170.12735036332309535No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT170.12735036332309535No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT160.11985916548056033No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG160.11985916548056033No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG150.11236796763802533No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT150.11236796763802533No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCAGAAGCG150.11236796763802533No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCTCCA150.11236796763802533No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGG140.1048767697954903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)