FastQCFastQC Report
Tue 7 May 2024
EGAF00007823299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13791
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCT4923.567544050467696No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCG4353.154231020230585No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1881.3632078892031034No Hit
ATTGCGCAATGTCAATACGGGGAGACACGGCCCAGACTCCTACGGGAGGC1831.3269523602349358No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1741.261692408092234No Hit
ATTGCGCAATGGATGACTCGGGGGTTCCAGGAAATAGCCCCTCCATTATA1220.8846349068232905No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1110.8048727430933217No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG720.5220796171416141No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCT700.507577405554347No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGAT670.48582408817344647No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC520.3770575012689435No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC510.36980639547531No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT450.3262997607135088No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT330.23928649118990647No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC330.23928649118990647No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT320.23203538539627294No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC310.22478427960263941No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCG290.21028206801537233No Hit
ATTGCGCAATGTGATGTATGGGGACGCGGAACGAGGCGAGGGACAAGGAA260.18852875063447175No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGATCT260.18852875063447175No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG250.18127764484083825No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG250.18127764484083825No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGAGCT240.1740265390472047No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA240.1740265390472047No Hit
ATTGCGCAATGCAGACGGGGGGGCCGGAATATATTAGCATGGAATAATAA230.16677543325357116No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCTGAGCG230.16677543325357116No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC230.16677543325357116No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC220.15952432745993764No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGCGGATCG220.15952432745993764No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG210.1522732216663041No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGTGC210.1522732216663041No Hit
ATTGCGCAATGCTATAGGGGGGGGGCACAGGCCAGGGGGTAGCGACTGTT210.1522732216663041No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA190.13777101007903705No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT180.13051990428540353No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG160.11601769269813647No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC160.11601769269813647No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA150.10876658690450293No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT150.10876658690450293No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG140.1015154811108694No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)