FastQCFastQC Report
Tue 7 May 2024
EGAF00007823300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13791
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTA9626.9755637734754545No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2391.7330142846784136No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1621.1746791385686317No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.8193749546805887No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGG1050.7613661083315205No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.6018417808715829No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGG790.5728373576970488No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTG660.47857298237981294No Hit
CTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTC640.4640707707925459No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGGGTA530.384308607062577No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT520.3770575012689435No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGT500.3625552896816765No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG420.3045464433326082No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC390.28279312595170764No Hit
CATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGGTCGCC370.2682909143644406Illumina Single End PCR Primer 1 (95% over 23bp)
ATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGG330.23928649118990647No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC330.23928649118990647No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGTTCTCGG320.23203538539627294No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA280.2030309622217388No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG280.2030309622217388No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC270.1957798564281053No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC270.1957798564281053No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.1957798564281053No Hit
GGGTTGAGCCCCGGGCTTTCACCTCTGACTTAAAAAACCGCCTACGCGCG260.18852875063447175No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.1740265390472047No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG230.16677543325357116No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT230.16677543325357116No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.16677543325357116No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGT220.15952432745993764No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTG220.15952432745993764No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG210.1522732216663041No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.1522732216663041No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA210.1522732216663041No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC210.1522732216663041No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGA200.14502211587267058No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA200.14502211587267058No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG200.14502211587267058No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA180.13051990428540353No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC180.13051990428540353No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTT170.12326879849177No Hit
CATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGATCTCGGTGGGCGCC160.11601769269813647No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC160.11601769269813647No Hit
CTCTTATACACATCTGACGCTGCCGACGATCCGTCTTGGGTGTAGTTCTC150.10876658690450293No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA150.10876658690450293No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA140.1015154811108694No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)