FastQCFastQC Report
Tue 7 May 2024
EGAF00007823306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2396
Total Bases361.7 kbp
Sequences flagged as poor quality0
Sequence length151
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT35214.691151919866444No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1245.175292153589315No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC371.5442404006677797No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGG110.4590984974958264No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTC80.333889816360601No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGGTGGTCGCC80.333889816360601Illumina RNA PCR Primer (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGT80.333889816360601No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA70.2921535893155259No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGGGGGGGGG70.2921535893155259No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGGGGGGGGGG60.25041736227045075No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG60.25041736227045075No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTGGGGGG60.25041736227045075No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGGGGGGGGGGGGG50.20868113522537562No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTGTGGG50.20868113522537562No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC50.20868113522537562No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGGCGGGGGGGGGG50.20868113522537562No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG50.20868113522537562No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG50.20868113522537562No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.20868113522537562No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTAGATCTCGGTGG50.20868113522537562No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGA50.20868113522537562No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTGGTGGGGGGGGGGG40.1669449081803005No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGGGGGGGGGC40.1669449081803005No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGGGGGGGGGGGG40.1669449081803005No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTCGGGGGGGGGG40.1669449081803005No Hit
CCTCTTGCTGGATCTGCCGGCACAGCTCCTCTCGCTTCTGACACAACTCC40.1669449081803005No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGGCGGGGGGGGGG40.1669449081803005No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTCGGGGG40.1669449081803005No Hit
ATCTTGAGGTAGGCGGCCTCGGTTCTGCGATGGTCCGGTCAAACTCGTTG40.1669449081803005No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTT40.1669449081803005No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGATCTC30.12520868113522537No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.12520868113522537No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGTGGG30.12520868113522537No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.12520868113522537No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGGCGGGGGGGGGG30.12520868113522537No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTGTG30.12520868113522537No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGGGGTGTGGGGGG30.12520868113522537No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAATGTA30.12520868113522537No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG30.12520868113522537No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG30.12520868113522537No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAA30.12520868113522537No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.12520868113522537No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAG30.12520868113522537No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGAGTTGTGGGGGGGGGGG30.12520868113522537No Hit
CATCTGACGCTGCCGACGATGACGGTGAGGTGGGGTTGTGGGGGGGGGGG30.12520868113522537No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTA30.12520868113522537No Hit
CTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGG30.12520868113522537No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.12520868113522537No Hit
CTCTTATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGTTGTG30.12520868113522537No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGGTGGTGTGGGGGG30.12520868113522537No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA30.12520868113522537No Hit
ATACACATCTGACGCTGCCGACGATGACGGTGAGGTGTTGTTGTCGGGGG30.12520868113522537No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAAATT30.12520868113522537No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC30.12520868113522537No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)