FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773
Sequences flagged as poor quality0
Sequence length151
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC20411.505922165820643No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC583.2712915961646925No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG221.2408347433728144No Hit
ATTGCGCAATGAGCAAGAGGGGGGCAAGGCTCTCGATGTGATTACCGAAG130.7332205301748449No Hit
ATTGCGCAATGTGAGGCGGCGGGGGGCGCCCTGTTCGAGCCTGTTTGGTG100.5640157924421884No Hit
ATTGCGCAATGCGTCAGGGGGGATGGTCAGAGTGAAGGTGAAGTAAAATT90.5076142131979695No Hit
ATTGCGCAATGCTGGTTGAGGGGAGTGGACAGCATGTCGGTCGCGGCGAC70.39481105470953193No Hit
ATTGCGCAATGTTACTAAAGGGATGGAAGCGAAAAGAATTGAGAAAAGGG70.39481105470953193No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.338409475465313No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.338409475465313No Hit
ATTGCGCAATGCATTTTGAGGGATGGCGTTTTAATGCTGGACATGGCTCG60.338409475465313No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC50.2820078962210942No Hit
ATTGCGCAATGCTTCGGGCGGAGGTGGGGCAGCAGCATGGCCAGCCAGGC50.2820078962210942No Hit
GGGGGCTGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.2820078962210942No Hit
ATTGCGCAATCTGTCTCTTATACACCGGTCTCTTATACACATCTCCGAGC40.2256063169768754No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTAAACACATCTCCGAGC40.2256063169768754No Hit
GGGGGGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.1692047377326565No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGATC30.1692047377326565No Hit
ATTGCGCAATCTGTCTCTTATACACCAGTCTCTTATACACATCTCCGAGC30.1692047377326565No Hit
ATTGCGCAATCTGGCTCTTATACACCTGTCTCTTATACACATCTCCGAGC30.1692047377326565No Hit
GGGCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.1692047377326565No Hit
ATTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.1692047377326565No Hit
ATTGCGCAATCTGTCTCTTATACACACTGTCTCTTATACACATCTCCGAG20.1128031584884377No Hit
ATTGCGCAATGGACTTCCCGGGATTAACATTGCGCAATCTGTCTCTTATA20.1128031584884377No Hit
GGGGTTTGGGTGGGGGGGGTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1128031584884377No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTATCTTATACACATCTCCGAGC20.1128031584884377No Hit
GCGTGCGGGCTGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1128031584884377No Hit
ATTGCGCAATCTGTCTCTTATACACATGTCTCTTATACACATCTCCGAGC20.1128031584884377No Hit
GGGCGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1128031584884377No Hit
GCGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1128031584884377No Hit
ATTGCGCAAACTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC20.1128031584884377No Hit
ATTGCGCAAGCAGAAGACGGCATACGAGATTCTGACTGTGACTGGAGTTC20.1128031584884377RNA PCR Primer, Index 40 (96% over 29bp)
ATTGCGCAATCTGTCTCTTATACACCTGTCTCGTATACACATCTCCGAGC20.1128031584884377No Hit
GTGTTTTGGCGGGGGGGGGCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1128031584884377No Hit
ATTGCGCAATCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA20.1128031584884377No Hit
GCGTGCGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1128031584884377No Hit
ATTGCGCAATATGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC20.1128031584884377No Hit
GGGGTCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1128031584884377No Hit
GGGGTGTGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1128031584884377No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAATCG20.1128031584884377No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTAATCT20.1128031584884377No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTACGAGC20.1128031584884377No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGACACCGTCATCG20.1128031584884377No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC20.1128031584884377No Hit
GGGGTGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1128031584884377No Hit
GGGGGATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1128031584884377No Hit
GGGGGCGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1128031584884377No Hit
GGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1128031584884377No Hit
ATTGCGCAATCTGTCTATTATACACATCCTGTCTCTTATACACATCTCCG20.1128031584884377No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATAAACATCTCCGAGC20.1128031584884377No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAC20.1128031584884377No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCACTTATACACATCTCCGAGC20.1128031584884377No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCGCTTATACACATCTCCGAGCCC20.1128031584884377No Hit
GGGGGAGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1128031584884377No Hit
ATTGCGCAATCTGTCTCTTATACACTGTCTCTTATACACATCTCCGAGCC20.1128031584884377No Hit
ATTGCGCAATCTGTCTCTTATACACGTGTCTCTTATACACATCTCCGAGC20.1128031584884377No Hit
ATTGCGCAATATGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC20.1128031584884377No Hit
GGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1128031584884377No Hit
CGTCTGTTGTGTATAAGAGACAGATTGCGCAATCCTGTCTCTTATACACA20.1128031584884377No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.1128031584884377No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)