FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007823310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773
Sequences flagged as poor quality0
Sequence length151
%GC64

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT29616.694867456288776No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1126.316976875352511No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC291.6356457980823462No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGTTGTTGTGGGGGGGGGGG60.338409475465313No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2256063169768754No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2256063169768754No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATCTCGG40.2256063169768754No Hit
TGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGC30.1692047377326565No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGGGGTGTTGTGGGGGGGGGGG30.1692047377326565No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT30.1692047377326565No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATCTCGGTGGTCGCC30.1692047377326565Illumina RNA PCR Primer (95% over 22bp)
GGTTTTATAAGCTGAAAATAAAGATCGTTTTTATAGCCTTGAAGTTCTTT30.1692047377326565No Hit
GCCTGTCCCAGCATAGAGGCCGTCGATTGCTGCTGCAAGTGTGACCGGAA30.1692047377326565No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA30.1692047377326565No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1692047377326565No Hit
GTTGTGTACACCACACGACCATGCTCTGGCCCACATGCGTACACGACACA30.1692047377326565No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGTTGTTGTGGG30.1692047377326565No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG30.1692047377326565No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCT20.1128031584884377No Hit
GGTAAAAGGCGTCGAATGCACCGGTACTGAATGGTGTCATTTTCGTGCTC20.1128031584884377No Hit
TTGTACCATAGACCAACAGGCCACCACAGAGCGTCAGGACGGCGGCTGTG20.1128031584884377No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGTAGGGGGGGGGG20.1128031584884377No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGTTTTTGGCGGGGGGGGGG20.1128031584884377No Hit
GTGTATAAGAGACAGATTGCGCAATGTTAATCCCGGGAAGTCCATTGCGC20.1128031584884377No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20.1128031584884377No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA20.1128031584884377No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1128031584884377No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGTTGTGGTGGG20.1128031584884377No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATATCGG20.1128031584884377No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGTGGG20.1128031584884377No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGTGGGGGGGGGGC20.1128031584884377No Hit
GGTACAGGCTTGGCGTGACGACATTCAGCGCACAATGGCAAGTCTCCGCG20.1128031584884377No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGTTTTTGTGGGGGGGGGGG20.1128031584884377No Hit
GATTCACACCAAACTTCACCTCGCAGGATCGTCGAGCTATTGCGTTCCGG20.1128031584884377No Hit
CATGCGTACACGACACATTCCTGCAGCATTCCACGATCCATCTTGACCCG20.1128031584884377No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA20.1128031584884377No Hit
GCGTCAGGACGGCGGCTGTGTCCGTGATGATGCCCGTGTGCGCCAGCACG20.1128031584884377No Hit
GCCCTAGGCCAGTCAGTGCTCTACCTCCTTAAATCTTTGATCATAGGCTA20.1128031584884377No Hit
ATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTGGGGGGTGG20.1128031584884377No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAAATCTTTA20.1128031584884377No Hit
GTATAAGAGACAGATTGCGCAATGCGCTCCCCGAGTTAACATTGCGCAAT20.1128031584884377No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGTGTTGTTGTAGGGGGTGGGC20.1128031584884377No Hit
TGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTG20.1128031584884377No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA20.1128031584884377No Hit
GTGTATAAGAGACAGATTGCGCAATGCCACCCCAGGGTCTCCATTGCGCA20.1128031584884377No Hit
ATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATCTCGGTGG20.1128031584884377No Hit
GGCGCATCGGCCTCGAATCGATCCGACGTGGGATCGAAGTAGACGACGCG20.1128031584884377No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATC20.1128031584884377No Hit
ATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGGTGTGGGGGG20.1128031584884377No Hit
CTTTCGGGCGTATTATATCCCACATTGCTTTCCACACGCGACTTCACCCA20.1128031584884377No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1128031584884377No Hit
ATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGTGGGTGG20.1128031584884377No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAA20.1128031584884377No Hit
ATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTTGGTGTCGGGGG20.1128031584884377No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1128031584884377No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGGTGTGGGGGGGGGGG20.1128031584884377No Hit
CTCGTGCACCGCCTGACCTGAGAGGACACGCGGAATGGCAGCTAGAATAT20.1128031584884377No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGTGTATATCTCGGTGGTCGCC20.1128031584884377Illumina Single End PCR Primer 1 (95% over 21bp)
CAGTAGCACCGCTATGGCAGGGACCACGGCTGATGTACCAAGGAAGCTTG20.1128031584884377No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC20.1128031584884377No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACAACTGACGCT20.1128031584884377No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG20.1128031584884377No Hit
AGTAAATACATTGAATCGCATTTATTCACTCGATGGGGCTTCGTTCTCTT20.1128031584884377No Hit
GTATGGTACATCGGCTGCTGATGCACACAAGTCACGGGAGGAAAGCGATG20.1128031584884377No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20.1128031584884377No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC20.1128031584884377No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTCTCGGGGGGGGGC20.1128031584884377No Hit
TTGATAGGCTTTGGTACGGTTAGCCGCCCTAGGCCAGTCAGTGCTCTACC20.1128031584884377No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)