FastQCFastQC Report
Tue 7 May 2024
EGAF00007823314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3280
Total Bases495.2 kbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1444.390243902439024No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC822.5No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.7621951219512195No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC200.6097560975609756No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.548780487804878No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC160.4878048780487805No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGT150.45731707317073167No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG150.45731707317073167No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG110.3353658536585366No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC100.3048780487804878No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGTT90.274390243902439No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG80.24390243902439024No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG80.24390243902439024No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG80.24390243902439024No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGA80.24390243902439024No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATCTCGG70.2134146341463415No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC70.2134146341463415No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCT70.2134146341463415No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA70.2134146341463415No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGGGTT60.1829268292682927No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATCTCGGTGGTCGCC60.1829268292682927Illumina RNA PCR Primer (95% over 22bp)
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA60.1829268292682927No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGTGGGGGGGGGGG60.1829268292682927No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA60.1829268292682927No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA60.1829268292682927No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC50.1524390243902439No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC50.1524390243902439No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA50.1524390243902439No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG50.1524390243902439No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTA50.1524390243902439No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGGGGGGGGGGGGG50.1524390243902439No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA50.1524390243902439No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGGGGT40.12195121951219512No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGGTGTGGGGGGGGGGG40.12195121951219512No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC40.12195121951219512No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGGGTTGTTGTGGG40.12195121951219512No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA40.12195121951219512No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCAA40.12195121951219512No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC40.12195121951219512No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA40.12195121951219512No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGTGGT40.12195121951219512No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)