FastQCFastQC Report
Tue 7 May 2024
EGAF00007823318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3278
Total Bases494.9 kbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT1655.033557046979865No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC902.7455765710799267No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT511.5558267236119585No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391.1897498474679682No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC170.5186089078706528No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.4575960951799878No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG140.4270896888346553No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG130.39658328248932273No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA130.39658328248932273No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.39658328248932273No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC120.36607687614399026No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC120.36607687614399026No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC110.33557046979865773No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCG90.2745576571079927No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGT80.24405125076266015No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG80.24405125076266015No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGA80.24405125076266015No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA70.21354484441732766No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG70.21354484441732766No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTT70.21354484441732766No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.21354484441732766No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGGGTT60.18303843807199513No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT60.18303843807199513No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA60.18303843807199513No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG60.18303843807199513No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA60.18303843807199513No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA60.18303843807199513No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGAC50.1525320317266626No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA50.1525320317266626No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA50.1525320317266626No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGTT50.1525320317266626No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC50.1525320317266626No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC50.1525320317266626No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA50.1525320317266626No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCT50.1525320317266626No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGA50.1525320317266626No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA40.12202562538133008No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCAAA40.12202562538133008No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGGGTA40.12202562538133008No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATCTCGGTGGTCGCC40.12202562538133008Illumina RNA PCR Primer (95% over 22bp)
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATCTGTA40.12202562538133008No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.12202562538133008No Hit
ATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.12202562538133008No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGTA40.12202562538133008No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAATTTA40.12202562538133008No Hit
CAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.12202562538133008No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC40.12202562538133008No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC40.12202562538133008No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTA40.12202562538133008No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAA40.12202562538133008No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATCTGTCT40.12202562538133008No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAATTT40.12202562538133008No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGT40.12202562538133008No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA40.12202562538133008No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA40.12202562538133008No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA40.12202562538133008No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)