FastQCFastQC Report
Tue 7 May 2024
EGAF00007823320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481
Total Bases223.6 kbp
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT15110.195813639432815No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC563.7812288993923024No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC151.012829169480081No Hit
GTGGAAAGAGAGTGGTCCAGAAATATAATTTATGTCCCGTCCTCTTACTC120.8102633355840648No Hit
TCCCACAACCCCGCACACGCAACGACTGTCGTCTATCACACGCCTGCGGT70.4726536124240378No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.4051316677920324No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.4051316677920324No Hit
GGCCGACAGACCTGAAAAGATGCATCCCACAACCCCGCACACGCAACGAC60.4051316677920324No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGTTGTGGGGGGGGGGG40.2700877785280216No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATCTCGGTGGTCGCC40.2700877785280216Illumina RNA PCR Primer (95% over 22bp)
CTCTTCATCCTTCTCGTGCCCCTCGTCAAAGATGCGGTCATGTGCATCAG40.2700877785280216No Hit
GTTCTGAGCCTATCATTGGTCCTGGTCGGTGATCCCTTCGTCAGTACGAG40.2700877785280216No Hit
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGC30.2025658338960162No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30.2025658338960162No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2025658338960162No Hit
GAATTTCTCGGGCTCCGCGCTACTCGGGAGAACATCAACACCTAGACTCT30.2025658338960162No Hit
GCCTATCATTGGTCCTGGTCGGTGATCCCTTCGTCAGTACGAGTACACGT30.2025658338960162No Hit
ATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATCTCGGTGG30.2025658338960162No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGTTTTTGTCGGGGGGGGGG30.2025658338960162No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2025658338960162No Hit
CCCTTAACCTTAATCAAAATCGGCATATCCTTAGGATCCTTGTCCTCCCA30.2025658338960162No Hit
GCAATAGCACTTTATCAGTTCCCCGATGCTAGAGCCTCAATCTCGCCAGG30.2025658338960162No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGT30.2025658338960162No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGTTGTTGTGGGGGGGGGGG30.2025658338960162No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC30.2025658338960162No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG30.2025658338960162No Hit
TCTTGAGGGTGTCGGCTTGCTGGGCCTGGAGCTTCTTCAGCTCCTCGGAG30.2025658338960162No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGC20.1350438892640108No Hit
ACTCGGGAGAACATCAACACCTAGACTCTTCCTTCACCTACGGGACTCTC20.1350438892640108No Hit
ATATATAAACTTTCTACTAAAATAGATTTCGCAGAATATCAGCTATCACC20.1350438892640108No Hit
GTATAAGAGACAGATTGCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1350438892640108No Hit
GTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1350438892640108No Hit
GCTTTTTCTACTCCATTCTGGTTGCGCATTTGTGCATGATGACTCTCAGA20.1350438892640108No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGTGTT20.1350438892640108No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAA20.1350438892640108No Hit
ATGTACGACAATGTCAGATCTTGGCTGATACACGCGGCGGCTCAAATTGC20.1350438892640108No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGGGGTGTTGTGGGGGGGGGGG20.1350438892640108No Hit
TCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCAA20.1350438892640108No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA20.1350438892640108No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTTTTGTGGGGGGGGGGG20.1350438892640108No Hit
CAACTACCCTATACATTCAGGTAGCGGCAACCCGACACAACTCGGGCTAG20.1350438892640108No Hit
GATCCGTAAATCTATTGGAAATGGAAATCAGTGCTATACAAAGAATCTTC20.1350438892640108No Hit
GAAGAGCACACGTCTGAACTCCAGTCACAGTCAGAATCTCGTATGCCGTC20.1350438892640108TruSeq Adapter, Index 13 (96% over 33bp)
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAATATG20.1350438892640108No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAAA20.1350438892640108No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGG20.1350438892640108No Hit
ACATACACTTAAGCAGCAAACTTGCTAATGGCGGCCTGGAGCTTCGATGG20.1350438892640108No Hit
CGTTAATACGCTTAATTATTCTACAGTGATACCCATGCTACGTGCAGTAC20.1350438892640108No Hit
GGCTTAGCAGGTGGTCCTGCCAGATTCACACGGAATTTCTCGGGCTCCGC20.1350438892640108No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGTTGTTGTCGGGGGGGGGG20.1350438892640108No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT20.1350438892640108No Hit
GCACTCTCACGAAGCCGTAATGGTAGAGGGACAAGGGGAGAGAGAGAGAG20.1350438892640108No Hit
CGACTGTCGTCTATCACACGCCTGCGGTTTAGCCTCTTCCCCGTTCGCTC20.1350438892640108No Hit
GCCATAGGCTTCTCTTCAGGTGCTTCAACAATGCAGCACTCTGATGTCGT20.1350438892640108No Hit
CATTATCCCATATCAATCGATTGTTCGTAAGGACCCAATTAGTGCTTAGT20.1350438892640108No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1350438892640108No Hit
ACCCCATAGAGCTCGGCGATCGATGATGATTCATCATTGGAAACACGATG20.1350438892640108No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1350438892640108No Hit
CACCTAGACTCTTCCTTCACCTACGGGACTCTCACCCACTCCGGTACCGC20.1350438892640108No Hit
GGTCAAAGTTAGCAGTGGCAATGGAGTCGAGGGCCTTCGTGCCCTTCAGT20.1350438892640108No Hit
CGTCACCACCAACTACCCTATACATTCAGGTAGCGGCAACCCGACACAAC20.1350438892640108No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGGTGTGTGGG20.1350438892640108No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGGGGTGTCGG20.1350438892640108No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGGTGGCGGGGGGGGGG20.1350438892640108No Hit
CTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGTGGGTGTG20.1350438892640108No Hit
GTTCAGAACATTCGCCTCCATACGCTATGTATTTACGTATGGATACTAGA20.1350438892640108No Hit
CTCATAGACAATATCAATTGTTTACCTTCTCTGTTACCTGCTTTTATCGT20.1350438892640108No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGGTGTGGGGGGGGGGG20.1350438892640108No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA20.1350438892640108No Hit
GACTGTCAGTTCATAAACAATAGATTCTTCTTCTGAGGTTAACTTCCAAT20.1350438892640108No Hit
CATCTGACGCTGCCGACGAGAAGACCGCGGGGTGGTTGTGGGGGGTGGGG20.1350438892640108No Hit
CTCCAGGCCGACAGACCTGAAAAGATGCATCCCACAACCCCGCACACGCA20.1350438892640108No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA20.1350438892640108No Hit
TCTGACGCTGCCGACGAGAAGACCGCTGTGTATATCTCGGTGGTCGCCGT20.1350438892640108Illumina Single End PCR Primer 1 (95% over 23bp)
CGCAACGACTGTCGTCTATCACACGCCTGCGGTTTAGCCTCTTCCCCGTT20.1350438892640108No Hit
GTATAAGAGACAGATTGCGCAATGGGGACCCCGGGATAGCATTGCGCAAT20.1350438892640108No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGTT20.1350438892640108No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG20.1350438892640108No Hit
GTCCAGCGGCCGGCGGTCGTGCTCGCGCAGGCACCGGATCACCTCGCCGC20.1350438892640108No Hit
TCCCACAACCCCGCACACGCAACGACTGTCGTCTATCACACGCGCACGGT20.1350438892640108No Hit
GATCTTGGCTGATACACGCGGCGGCTCAAATTGCATTGCGAGTGATTCCC20.1350438892640108No Hit
CTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTACTC20.1350438892640108No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATCTCGG20.1350438892640108No Hit
CCCTTGGCGTAGCCGACAATCTCGTCCCCTCCGAGTTTACTTTCACCATA20.1350438892640108No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA20.1350438892640108No Hit
CTCCGGTACCGCTTCCCAACGGATTCAGCTAGAAAAATCATCAGCGCTCC20.1350438892640108No Hit
GAAATATAATTTATGTCCCGTCCTCTTACTCATAGACAATATCAATTGTT20.1350438892640108No Hit
GAGCCTATCATTGGTCCTGGTCGGTGATCCCTTCGTCAGTACGAGTACAC20.1350438892640108No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA20.1350438892640108No Hit
GTGCGCACCACCTTCAGTGGATCCAGCACACCAGCAGCAATCAGGTCCTT20.1350438892640108No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)