FastQCFastQC Report
Tue 7 May 2024
EGAF00007823327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12856
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCG3913.0413814561294337No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCT3732.901369010578718No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3602.8002489110143123No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2221.7268201617921592No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCG850.6611698817672682No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG840.6533914125700062No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTAT800.6222775357809583No Hit
ATTGCGCAATGAACCAGTGGGGGTGTGGGGACAGCCCCGAGTTGCCGAAA720.5600497822028625No Hit
ATTGCGCAATGAAGTCTCAGGGCAAGGACGGGAGTCTTCAGTGTCCGTCG690.5367143746110765No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.49782202862476665No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGC580.45115121344119474No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCT560.43559427504667086No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT550.42781580584940887No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATAC510.39670192906036095No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT500.38892345986309895No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC470.36558805227131297No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT430.3344741754822651No Hit
ATTGCGCAATGAACTAGGGGGGGTACCGGGAGAATAAGCTCCGGCTAACT420.3266957062850031No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT370.2878033602986932No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA320.24891101431238333No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG310.24113254511512133No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG290.22557560672059737No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC290.22557560672059737No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG290.22557560672059737No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCC260.20224019912881147No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC250.19446172993154948No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC250.19446172993154948No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC230.17890479153702551No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG210.16334785314250155No Hit
ATTGCGCAATGTTGAAGCGAGGGCTGGGCCGGAAGCTCCGCTTTCTCTTC210.16334785314250155No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT190.1477909147479776No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC190.1477909147479776No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG190.1477909147479776No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT180.14001244555071563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT180.14001244555071563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG180.14001244555071563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT180.14001244555071563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT180.14001244555071563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT170.13223397635345363No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC160.12445550715619166No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT160.12445550715619166No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGC160.12445550715619166No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT140.10889856876166772No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCG140.10889856876166772No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTG140.10889856876166772No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130.10112009956440574No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTAGCG130.10112009956440574No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATA130.10112009956440574No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)