FastQCFastQC Report
Tue 7 May 2024
EGAF00007823336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3904
Total Bases589.5 kbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2807.172131147540983No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1473.765368852459016No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.7684426229508197No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.742827868852459No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC240.6147540983606558No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.4610655737704918No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG160.4098360655737705No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCG150.38422131147540983No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC150.38422131147540983No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG130.3329918032786885No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCT130.3329918032786885No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAG120.3073770491803279No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTT100.25614754098360654No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA100.25614754098360654No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.25614754098360654No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC100.25614754098360654No Hit
CATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATCTCGGTGGTCGCC90.2305327868852459Illumina RNA PCR Primer (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTA90.2305327868852459No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGA90.2305327868852459No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAG90.2305327868852459No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGGGTT80.20491803278688525No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT80.20491803278688525No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG80.20491803278688525No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA70.17930327868852458No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGC70.17930327868852458No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGT70.17930327868852458No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC70.17930327868852458No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG60.15368852459016394No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC60.15368852459016394No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG60.15368852459016394No Hit
CAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG50.12807377049180327No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGGGGT50.12807377049180327No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT50.12807377049180327No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC50.12807377049180327No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA50.12807377049180327No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGG50.12807377049180327No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA50.12807377049180327No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA50.12807377049180327No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA50.12807377049180327No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA50.12807377049180327No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATG40.10245901639344263No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAATT40.10245901639344263No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA40.10245901639344263No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.10245901639344263No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAA40.10245901639344263No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA40.10245901639344263No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCTGTGTAGATCTCGG40.10245901639344263No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATTTA40.10245901639344263No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGTTGTTGTGGG40.10245901639344263No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA40.10245901639344263No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATTT40.10245901639344263No Hit
CTTATACACATCTGACGCTGCCGACGAGAAGACCGCGGGGGTGGTGTGGG40.10245901639344263No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)