FastQCFastQC Report
Tue 7 May 2024
EGAF00007823351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10536
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCG4163.9483675018982534No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCT2952.7999240698557326No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2192.078587699316629No Hit
ATTGCGCAATGATCGTCGGGGGGAAGTATATTGTAATTGTTTAATCTTAG1881.7843583902809415No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1751.660971905846621No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1371.300303720577069No Hit
ATTGCGCAATGGATCCGAGGGGTGGCCAAATCCTCCATCCTTTTGGATGT890.8447228549734245No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATC780.7403189066059226No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG720.683371298405467No Hit
ATTGCGCAATGCGAACCGCGGGAGCAGCGCCTTGGAGGTCCCGTGGATCT720.683371298405467No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC600.5694760820045558No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCAT560.5315110098709187No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT530.503037205770691No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC470.44608959757023536No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAG420.3986332574031891No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCG420.3986332574031891No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT400.3796507213363705No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT360.3416856492027335No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG320.30372057706909644No Hit
ATTGCGCAATGACTACACCGGGGGGAGGCAGCAGTAGGGAATCTTCCGCA300.2847380410022779No Hit
ATTGCGCAATGTCATAGAGGGGGGAAGGGTATCACCGAAGTGAATACATA300.2847380410022779No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT290.2752467729688686No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC250.2372817008352316No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG250.2372817008352316No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG220.2088078967350038No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT210.19931662870159456No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC210.19931662870159456No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG200.18982536066818526No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAG190.180334092634776No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT190.180334092634776No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA190.180334092634776No Hit
ATTGCGCAATGACAGCCACTCTGGAGGAGAAACGGAAGGAAAAGGCCAAG190.180334092634776No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCAGCG190.180334092634776No Hit
ATTGCGCAATGGTACACAAGGGAAAGCGGGGGACCTTCGGGCCTCGCGCT180.17084282460136674No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTAGGCATCCATCT170.1613515565679575No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT170.1613515565679575No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTA160.15186028853454822No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA160.15186028853454822No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT160.15186028853454822No Hit
ATTGCGCAATGGTCTCCCAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG150.14236902050113895No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC150.14236902050113895No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT140.13287775246772968No Hit
ATTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.12338648443432042No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC130.12338648443432042No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT130.12338648443432042No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC130.12338648443432042No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC120.11389521640091116No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120.11389521640091116No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT120.11389521640091116No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT120.11389521640091116No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG110.1044039483675019No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG110.1044039483675019No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTG110.1044039483675019No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC110.1044039483675019No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA110.1044039483675019No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT110.1044039483675019No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTA110.1044039483675019No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGG110.1044039483675019No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)