FastQCFastQC Report
Tue 7 May 2024
EGAF00007823360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12730
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGTA5073.9827179890023565No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGTT2471.9402985074626864No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2451.9245875883739199No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGGA2071.6260801256873527No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1901.4925373134328357No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGGT1691.3275726630007856No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.9662215239591517No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.950510604870385No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTC1010.7934014139827181No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACTCGTG750.589159465828751No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG570.44776119402985076No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTA560.4399057344854674No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.3377847604084839No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA380.2985074626865672No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC360.28279654359780043No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC330.2592301649646504No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.2592301649646504No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG320.2513747054202671No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC310.24351924587588375No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG260.20424194815396698No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA250.19638648860958366No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG220.17282010997643363No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA220.17282010997643363No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA210.16496465043205027No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGT210.16496465043205027No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC200.15710919088766695No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA190.1492537313432836No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC190.1492537313432836No Hit
ACCTTAGGATGGTTATAGTTACCACCGCCGTTTACTGGGGCTTAAATTCA180.14139827179890022No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA180.14139827179890022No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC180.14139827179890022No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC180.14139827179890022No Hit
CTTATACACATCTGACGCTGCCGACGAACGTATACTCGTGTAGATCTCGG170.1335428122545169No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA160.12568735271013354No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACGTATACT160.12568735271013354No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA160.12568735271013354No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA150.1178318931657502No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.1178318931657502No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAT150.1178318931657502No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT140.10997643362136685No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA140.10997643362136685No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA130.10212097407698349No Hit
GTCCGTATACATCGTCTTACGACTTCGCACGGACCTGTGTTTTTAGTAAA130.10212097407698349No Hit
TTATTATACTGATATGGAAAAAGGATTTAGTACAGTTATGCTCAGATGAA130.10212097407698349No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)