FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007823369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007823369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16647
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATATCG6754.054784645882141No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATATCT3942.366792815522316No Hit
ATTGCGCAATGCACAGGACGGGTCAAGCCCGGCGTGCAGCCCTCAGGAGC2591.5558358863458883No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2421.4537153841533008No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2191.3155523517750947No Hit
ATTGCGCAATGGAGATGGAGGGGAGTACGGTCGCAAGATTAAAACTCAAA1550.931098696461825No Hit
ATTGCGCAATGGAGGGGCCGGGGCCTAGAATGTGGGTCAGGGAGAGTCAG1460.8770349011833964No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1450.8710278128191264No Hit
ATTGCGCAATGTCGTTTGAGGGGCTTTCTCTGCGGAATCACCATGGCGGC1340.8049498408121584No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATAT830.4985883342343966No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC790.47455998077731726No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG790.47455998077731726No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATATCG700.42049618549888873No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTA680.40848200877034896No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC630.3784465669489998No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC620.37243947858473No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT530.31837568330630145No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT400.24028353457079352No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT350.21024809274944437No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC320.19222682765663485No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC310.186219739292365No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA310.186219739292365No Hit
ATTGCGCAATGCATTACAGGGGGGGTCAAGTGAATAAGTGCATATGGTGG300.18021265092809513No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCC290.17420556256382533No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT280.16819847419955547No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC280.16819847419955547No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG250.15017720910674595No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA240.14417012074247612No Hit
ATTGCGCAATGATCCCAGGGGGGCGCAAGCTCTCGTGGGGGGCACAGGCC230.1381630323782063No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA220.13215594401393646No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC220.13215594401393646No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC210.1261488556496666No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT210.1261488556496666No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCGATATCG200.12014176728539676No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG200.12014176728539676No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTATATCT190.11413467892112694No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG190.11413467892112694No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG190.11413467892112694No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCTAGATCG180.10812759055685708No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCAGCT170.10212050219258724No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT170.10212050219258724No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)